中国癌症防治杂志2025，Vol.17Issue(6)：730-738,9.DOI:10.3969/j.issn.1674-5671.2025.06.11
M2型肿瘤相关巨噬细胞通过调控CTHRC1促进胃癌MKN-45细胞增殖及上皮-间质转化
M2-type tumor-associated macrophages promote the proliferation and epithelial-mesenchymal transition of gastric cancer MKN-45 cells by regulating CTHRC1 expression
摘要
Abstract
Objective To investigate the effects of M2-type tumor-associated macrophages(TAMs)on the proliferation of gastric cancer cells and epithelial-mesenchymal transition(EMT),along with possible mechanisms.Methods THP-1 cells were differentiated into M2-type macrophages in vitro and treated with conditioned medium from gastric cancer MKN-45 cells to obtain M2-type TAMs(M2-TAMs).M2-TAMs were co-cultured with MKN-45 cells(MKN-45 group and M2-TAMs/MKN-45 group).In addition,siRNA technology was used to silence collagen triple helix repeat containing 1(CTHRC1)in MKN-45 cells,the cells were co-cultured with or without M2-TAMs(si-NC group,si-CTHRC1 group,M2-TAMs/si-NC group,and M2-TAMs/si-CTHRC1 group).Cell proliferation,invasion and migration abilities were detected by CCK-8,colony formation,Transwell and scratch test.The levels of matrix metallopro-teinase 2(MMP2)and matrix metalloproteinase 9(MMP9)in cell supernatant were detected by ELISA.qRT-PCR was used to detect the expression level of CTHRC1 mRNA in cells.Western blot was used to detect the protein expression levels of E-cadherin,N-cadherin,Vimentin and CTHRC1.Results Compared with the MKN-45 group,the M2-TAMs/MKN-45 group showed significantly increased cell proliferation,migration and invasion abilities(all P<0.05),upregulated MMP2 and MMP9 levels(all P<0.01),decreased E-cadherin expression(P<0.001),and increased N-cadherin,Vimentin and CTHRC1 expression(all P<0.01).Compared with the si-NC group,the si-CTHRC1 group exhibited significantly reduced cell proliferation,migration and invasion abilities(all P<0.05),increased E-cadherin expression(P<0.001),and decreased CTHRC1,N-cadherin and Vimentin expression(all P<0.01).Compared with the M2-TAMs/si-NC group,the M2-TAMs/si-CTHRC1 group showed significantly decreased cell proliferation,migration and invasion abilities(all P<0.05),increased E-cadherin expression(P<0.001),and decreased CTHRC1,N-cadherin and Vimentin expression(all P<0.01).Conclusions M2-type TAMs may promote the proliferation and EMT of gastric cancer MKN-45 cells by upregulating CTHRC1 expression.关键词
肿瘤相关巨噬细胞/胃癌/胶原三股螺旋重复蛋白1/增殖/上皮-间质转化Key words
Tumor-associated macrophages/Gastric cancer/Collagen triple helix repeat containing 1/Proliferation/Epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
索菲娅,田丽丽,闫全志,苏日娜,邢宏军,赵慧英..M2型肿瘤相关巨噬细胞通过调控CTHRC1促进胃癌MKN-45细胞增殖及上皮-间质转化[J].中国癌症防治杂志,2025,17(6):730-738,9.基金项目
内蒙古自治区卫生健康委2023年首府地区公立医院高水平临床专科建设科研项目(2023SGGZ072-04) （2023SGGZ072-04）
北京大学肿瘤医院内蒙古医院"青苗"人才计划项目(QM202318) （QM202318）
