中国中医药信息杂志2026，Vol.33Issue(1)：64-69,6.DOI:10.19879/j.cnki.1005-5304.202506411
基于自噬探讨养阴益气方干预博来霉素诱导的大鼠特发性肺纤维化作用机制
Exploration on the Mechanism of Yangyin Yiqi Prescription in Bleomycin-induced Idiopathic Pulmonary Fibrosis in Rats Based on Autophagy
摘要
Abstract
Objective To observe the effects of Yangyin Yiqi Prescription on rats with pulmonary fibrosis induced by bleomycin;To explore the mechanism by which Yangyin Yiqi Prescription improving idiopathic pulmonary fibrosis through autophagy.Methods The rat model of idiopathic pulmonary fibrosis was established by bleomycin intratracheal instillation.The rats were randomly divided into blank group,model group,Yangyin Yiqi Prescription group,pirfenidone group and prednisone group.From the next day after modeling,each group was given corresponding drugs by gavage,and the blank group and model group were given equal volume of normal saline by gavage,once a day for 28 days.The maximum expiratory flow(PEF)and volume of tidal(VT)were detected by noninvasive ventilator,the morphology of lung tissue was observed by HE staining and Masson staining,the protein expressions of LC3BⅡ/Ⅰ,Beclin-1,p62 and MMP7 in lung tissue were detected by Western blot,the mRNA expressions of LC3B,Beclin-1 and p62 in lung tissue were detected by RT-PCR.Results Compared with the blank group,PEF and VT in the model group were significantly decreased(P<0.01),the alveolar wall thickened and deformed,the alveolar cavity narrowed or disappeared,the inflammatory cell infiltration was obvious,a large number of collagen fibers were deposited,and the alveolitis score and pulmonary fibrosis score significantly increased(P<0.01);the protein expressions of LC3BⅡ/Ⅰ and Beclin-1 in lung tissue decreased,while the protein expressions of p62 and MMP7 increased(P<0.01),the mRNA expressions of LC3B and Beclin-1 decreased,while the expression of p62 mRNA increased(P<0.01).Compared with the model group,PEF and VT in Yangyin Yiqi Prescription group significantly increased(P<0.01),inflammatory cell infiltration in lung tissue was reduced,collagen fiber deposition was reduced,alveolitis score and pulmonary fibrosis score significantly decreased(P<0.01);the protein expressions of LC3BⅡ/Ⅰ and Beclin-1 in lung tissue increased,while the protein expressions of p62 and MMP7 decreased(P<0.05,P<0.01),the mRNA expressions of LC3B and Beclin-1 increased,while the mRNA expression of p62 decreased(P<0.05,P<0.01).Conclusion Yangyin Yiqi Prescription may alleviate alveolar inflammation by up-regulating LC3BⅡ/Ⅰ and Beclin-1,down-regulating the expressions of p62 and MMP7,and delaying the production and accumulation of collagen fibers,in order to slowing down the progression of idiopathic pulmonary fibrosis.关键词
特发性肺纤维化/养阴益气方/自噬/p62/大鼠Key words
idiopathic pulmonary fibrosis/Yangyin Yiqi Prescription/autophagy/p62/rats分类
医药卫生引用本文复制引用
李辉,孟丽红,薛晓明,杨硕,谢地,王梦真,吴敏,杨卓敏,王晋煴..基于自噬探讨养阴益气方干预博来霉素诱导的大鼠特发性肺纤维化作用机制[J].中国中医药信息杂志,2026,33(1):64-69,6.基金项目
国家自然科学基金(82205079) （82205079）
山西省基础研究计划(202203021212068) （202203021212068）
山西省人社厅博士基金项目(2023年) （2023年）
