中国中药杂志2026，Vol.51Issue(2)：565-578,14.DOI:10.19540/j.cnki.cjcmm.20251010.204
基于UHPLC-ESI-Q-TOF-MS/MS和UHPLC-QQQ-MS/MS的栀子水提物代谢轮廓分析和药代动力学研究
Metabolic profiling and pharmacokinetic studies of Gardeniae Fructus aqueous extracts by UHPLC-ESI-Q-TOF-MS/MS and UHPLC-QQQ-MS/MS
摘要
Abstract
Gardeniae Fructus(GF)is one of the most commonly used medicinal and edible resources in China,renowned for its anti-inflammatory and antipyretic effects.However,studies of the chemical characteristics,metabolic transformation and pharmacokinetic behavior of GF in vivo remain incomplete.This study utilized mass defect filtering(MDF)combined with UHPLC-ESI-Q-TOF-MS/MS to analyze the in vitro chemical constituents of GF aqueous extracts and its metabolites in vivo in rats,comprehensively characterizing 114 chemical constituents and 181 endogenous substances,including 18 prototype constituents and 163 metabolites(21 phase Ⅰ and 142 phase Ⅱ metabolites).Additionally,a quantitative method using UHPLC-QQQ-MS/MS was developed to analyze the in vivo dynamics of seven bioactive compounds,and the pharmacokinetic parameters of the main active components in the GF aqueous extracts,namely geniposidic acid,deacetyl asperulosidic acid methyl ester,gardenoside,genipin,genipin 1-β-D-gentiobioside,geniposide,and crocetin,were obtained.This study conducted a comprehensive and systematic investigation into the potential material basis and the in vivo biotransformation profile of GF aqueous extracts based on UHPLC-ESI-Q-TOF-MS/MS coupled with MDF technology and UHPLC-QQQ-MS/MS,providing crucial evidence for further elucidating its pharmacological mechanisms.关键词
栀子水提物/代谢轮廓/药代动力学/UHPLC-ESI-Q-TOF-MS/MS/UHPLC-QQQ-MS/MSKey words
Gardeniae Fructus aqueous extracts/metabolic profiling/pharmacokinetics/UHPLC-ESI-Q-TOF-MS/MS/UHPLC-QQQ-MS/MS引用本文复制引用
何江,史雯馨,王诗琪,龚普阳,洪怡,郭瑜婕..基于UHPLC-ESI-Q-TOF-MS/MS和UHPLC-QQQ-MS/MS的栀子水提物代谢轮廓分析和药代动力学研究[J].中国中药杂志,2026,51(2):565-578,14.基金项目
国家自然科学基金青年科学基金项目(82204750) （82204750）
