中国中医药信息杂志2026，Vol.33Issue(1)：70-76,7.DOI:10.19879/j.cnki.1005-5304.202505315
"三法三穴"推拿对坐骨神经损伤大鼠骨骼肌能量代谢和线粒体动力学的影响
Effects of"Three Methods and Three Acupoints"Massage on Energy Metabolism and Mitochondrial Dynamics in Skeletal Muscle of Rats with Sciatic Nerve Injury
摘要
Abstract
Objective To analyze the improvement mechanism of motor dysfunction after sciatic nerve injury(SNI)through"three methods and three acupoints"massage from the perspective of energy metabolism and mitochondrial dynamics in skeletal muscle.Methods A total of 36 SD rats were selected and randomly divided into blank group,sham-operation group,model group and massage group,with 9 rats in each group.The SNI model was constructed by clamping method in the model group and massage group;only the sciatic nerve was exposed in the sham-operation group without clamping operation,while the blank group did not receive any treatment.Before modeling,after 10 and 20 times of intervention,the rats were subjected to the inclined plate test to assess their motor functions.After intervention,the gastrocnemius muscle tissue was taken from the rats,and its histomorphology was observed by HE staining,the contents of adenosine triphosphate(ATP)in gastrocnemius muscle tissue was determined by colorimetric assay,immunofluorescence staining was used to detect the expressions of OPA1,DRP1,MFN1 and FIS1 in gastrocnemius muscle tissue,the mRNA expressions of OPA1,MFN2 and DRP1 in gastrocnemius muscle tissue were detected by real-time PCR,and the protein expressions of OPA1,MFN2 and DRP1 in gastrocnemius muscle tissue were detected by Western blot.Results Compared with the blank group and sham-operation group,the angle of the inclined plate test of the rats in the model group was significantly reduced(P<0.01),indicating that the muscle strength of the hind limb was weakened;gastrocnemius muscle fibers arranged irregularly,gaps widened,and nuclei distributed irregularly;the content of ATP in gastrocnemius muscle tissue significantly decreased(P<0.01),the mRNA expressions of OPA1 and MFN2 significantly decreased,the mRNA expression of DRP1 significantly increased,the protein expressions of OPA1,MFN1 and MFN2 significantly decreased(P<0.01),and the protein expressions of DRP1 and FIS1 significantly increased(P<0.01).Compared with the model group,the inclined plate test angle of rats in massage group significantly increased(P<0.01),indicating that the muscle strength of the hind limb was improved;gastrocnemius muscle fibers were more regularly arranged,fiber gaps were narrowed,and the distribution of cell nuclei tended to be uniform,the ATP content in gastrocnemius muscle tissue significantly increased(P<0.01),the mRNA expressions of OPA1 and MFN2 significantly increased,the mRNA expression of DRP1 significantly decreased(P<0.01),the protein expressions of OPA1,MFN1 and MFN2 significantly increased,whereas the protein expressions of DRP1 and FIS1 significantly decreased(P<0.01).Conclusion The"three methods and three acupoints"massage can significantly improve the morphology and structure of the skeletal muscle in SNI rats and recover their motor dysfunction.The mechanism may be closely related to the regulation of mitochondrial dynamics imbalance in skeletal muscle and the enhancement of ATP content in skeletal muscle.关键词
三法三穴/坐骨神经损伤/线粒体动力学/肌萎缩/运动功能障碍/大鼠Key words
three methods and three acupoints/sciatic nerve injury/mitochondrial dynamics/muscular atrophy/motor dysfunction/rats分类
医药卫生引用本文复制引用
张弘正,许悦,于天源,闫家旺,娜仁图雅,张英琦,刘家玥,张汉钰,孙佳伟.."三法三穴"推拿对坐骨神经损伤大鼠骨骼肌能量代谢和线粒体动力学的影响[J].中国中医药信息杂志,2026,33(1):70-76,7.基金项目
国家自然科学基金(82274675) （82274675）
北京市自然科学基金(7232278) （7232278）
