中国中药杂志2026，Vol.51Issue(1)：73-82,10.DOI:10.19540/j.cnki.cjcmm.20250929.401
茅苍术Aux/IAA基因家族鉴定及其对生长素的响应分析
Identification of Aux/IAA gene family in Atractylodes lancea and their response to auxin
摘要
Abstract
Using the whole-genome data of Atractylodes lancea,members of the auxin(Aux)/indole-3-acetic acid(IAA)gene family were identified and characterized,and the effects of 1-naphthaleneacetic acid(NAA)treatment on the expression levels of this gene family were investigated,providing a foundation for further studies on the functions of the A.lancea Aux(AlAux)/IAA gene family.Members of the AlAux/IAA gene family were identified from the A.lancea whole-genome data using TBtools-Ⅱ.Bioinformatics software was employed to analyze the physicochemical properties of the encoded proteins,construct a phylogenetic tree,and predict conserved motifs and gene structures.Tissue-cultured seedlings of A.lancea were used as experimental materials,and key genes of the AlAux/IAA gene family were screened by transcriptome sequencing.The expression of key AlAux/IAA genes was analyzed after one month of NAA treatment.A total of 53 AlAux/IAA genes were identified from the A.lancea genome.Most AlAux/IAA proteins were localized in the nucleus,lacked transmembrane domains,and exhibited negative hydrophobicity.Among them,44 AlAux/IAA genes were predicted to encode functional Aux/IAA transport proteins.Transcriptome sequencing and RT-qPCR analyses showed that the AlAux/IAA family genes exhibited spatiotemporal expression specificity,and different members displayed varying response times and intensities to NAA treatment.Tissue-specific expression analysis revealed that the AlAux/IAA gene family was generally highly expressed in flowers.This study identified the Aux/IAA gene family members in A.lancea,preliminarily analyzed their sequence characteristics,and examined their expression features in response to Aux.It is speculated that AlAux/IAA13 and AlAux/IAA38 may be involved in rooting and bud growth and development during the subculture process of A.lancea tissue-cultured seedlings.These results provide a theoretical basis for elucidating the molecular mechanisms of AlAux/IAA gene function and Aux-mediated regulation of rhizome growth in A.lancea.关键词
茅苍术/Aux/IAA基因家族/全基因组鉴定/基因表达/生物信息学Key words
Atractylodes lancea/Aux/IAA gene family/genome-wide identification/gene expression/bioinformatics引用本文复制引用
陈雷,何梓茉,陈袁袁,孙琳棋,王萌,黄晓,邓娟,李谦,余坤..茅苍术Aux/IAA基因家族鉴定及其对生长素的响应分析[J].中国中药杂志,2026,51(1):73-82,10.基金项目
湖北省重点研发计划项目(2023BBB151) （2023BBB151）
湖北省高等学校优秀中青年科技创新团队计划项目(T2022020) （T2022020）
湖北省教育厅青年项目(Q20222013) （Q20222013）
中药资源与中药化学湖北省重点实验室开放基金项目(KLRCCM2409) （KLRCCM2409）
