中国药房2026，Vol.37Issue(2)：174-179,6.DOI:10.6039/j.issn.1001-0408.2026.02.07
益肾排毒方调控ROS/TXNIP/NLRP3通路对慢性肾衰竭大鼠肾纤维化的影响
Effects of Yishen paidu formula on renal fibrosis in rats with chronic renal failure by regulating the ROS/TXNIP/NLRP3 pathway
摘要
Abstract
OBJECTIVE To investigate the effects and mechanism of the Yishen paidu formula on renal fibrosis in rats with chronic renal failure(CRF)through the reactive oxygen species(ROS)/thioredoxin-interacting protein(TXNIP)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)pathway.METHODS Rats were randomly divided into control group,model group,Yishen paidu formula low-dose(Yishen paidu formula-L)group,Yishen paidu formula high-dose(Yishen paidu formula-H)group,Yishen paidu formula-H+pcDNA-NC group,and Yishen paidu formula-H+pcDNA-TXNIP group,with 10 rats in each group.Except for control group,all other rats were fed a diet containing 0.5%adenine to establish a CRF model;the rats were then administered corresponding drugs or normal saline intragastrically or via tail vein,once daily,for 8 consecutive weeks.After the last administration,the levels of serum creatinine(Scr),blood urea nitrogen(BUN),ROS,superoxide dismutase(SOD),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-1β were measured in each group.Pathological changes in renal tissue were observed,and the protein expression levels of Collagen Ⅲ,α-smooth muscle actin(α-SMA),transforming growth factor-β1(TGF-β1),TXNIP and NLRP3 in renal tissue were detected.RESULTS Compared with model group,the renal histopathological damage and fibrosis of rats in Yishen paidu formula-L group and Yishen paidu formula-H group were significantly alleviated.The levels of Scr,BUN,ROS,MDA,TNF-α,IL-6 and IL-1β,and the protein expressions of Collagen Ⅲ,α-SMA,TGF-β1,TXNIP and NLRP3 were significantly decreased,while SOD levels were significantly increased(P＜0.05).Moreover,the changes were more pronounced in the Yishen paidu formula-H group(P＜0.05).Compared with Yishen paidu formula-H+pcDNA-NC group,above indexes of rats in Yishen paidu formula-H+pcDNA-TXNIP group were reversed significantly(P＜0.05).CONCLUSIONS Yishen paidu formula can inhibit renal fibrosis in CRF rats by suppressing the ROS/TXNIP/NLRP3 pathway.关键词
益肾排毒方/慢性肾衰竭/肾纤维化/ROS/TXNIP/NLRP3通路Key words
Yishen paidu formula/chronic renal failure/renal fibrosis/ROS/TXNIP/NLRP3 pathway分类
医药卫生引用本文复制引用
冯立,彭博文,彭斌,冯雪,朱双益,熊玮,胡溪,孙小慧..益肾排毒方调控ROS/TXNIP/NLRP3通路对慢性肾衰竭大鼠肾纤维化的影响[J].中国药房,2026,37(2):174-179,6.基金项目
武汉市知识创新专项(No.2023020201020556) （No.2023020201020556）
武汉市中医药科研项目(No.WZ22Q51) （No.WZ22Q51）
