中国药房2026，Vol.37Issue(2)：186-191,6.DOI:10.6039/j.issn.1001-0408.2026.02.09
茯苓酸调控Sirt1/PGC-1α通路改善妊娠高血压大鼠肾损伤的机制
Mechanism of pachymic acid in ameliorating renal injury in pregnancy induced hypertension rats by regulating the Sirt1/PGC-1α pathway
摘要
Abstract
OBJECTIVE To investigate the mechanism of pachymic acid on renal injury in pregnancy induced hypertension(PIH)rats by regulating the silent information regulator transcript 1/peroxisome proliferator-activated receptor γ coactivator-1α(Sirt1/PGC-1α)pathway.METHODS Pregnant SD rats were prepared by co-caging and PIH model was induced using N-nitro-L-arginine methyl ester(L-NAME)method.PIH rats were randomly divided into model group,L-pachymic acid(low-dose pachymic acid,10 mg/kg)group,H-pachymic acid(high-dose pachymic acid,20 mg/kg)group,and H-pachymic acid+EX527(20 mg/kg pachymic acid+10 mg/kg EX527)group,with 6 rats in each group.Another 6 normal pregnant rats were selected as blank group.Each group was given relevant medicine or solvent intragastrically or intraperitoneally daily,once a day,for 28 consecutive days.After the last administration,24 h urinary protein and tail artery systolic blood pressure(SBP)were measured in pregnant rats from each group,along with the levels of serum creatinine(Scr),blood urea nitrogen(BUN),uric acid(UA),and cystatin C(Cys-C).The contents of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),and 8-hydroxy-2′-deoxyguanosine(8-OHdG)in renal tissue,as well as the mRNA and protein expression levels of Sirt1 and PGC-1α,were also determined.Meanwhile,renal histopathological changes in rats from each group were evaluated using hematoxylin-eosin(HE)staining and periodic acid-Schiff(PAS)staining.RESULTS Compared with model group,L-pachymic acid group and H-pachymic acid group exhibited significant decreases in 24 h urine protein quantification,tail artery SBP,Scr,BUN,UA,Cys-C levels,glomerulosclerosis index score of renal tissue,renal tubular injury score,the percentage of PAS positive area,MDA and 8-OHdG(P＜0.05).Conversely,the contents of SOD and GSH-Px,along with the mRNA and protein expression levels of Sirt1 and PGC-1α,were significantly increased(P＜0.05).Moreover,these improvements were more pronounced in H-pachymic acid group(P＜0.05).Compared with H-pachymic acid group,the aforementioned indicators in pregnant rats from the H-pachymic acid+EX527 group showed significant reversal(P＜0.05).CONCLUSIONS Pachymic acid significantly ameliorates renal injury induced by PIH in rats,potentially through activation of the Sirt1/PGC-1α pathway.关键词
茯苓酸/妊娠高血压/肾损伤/Sirt1/PGC-1α通路Key words
pachymic acid/pregnancy induced hypertension/renal injury/Sirt1/PGC-1α pathway分类
医药卫生引用本文复制引用
朱俊江,林金秤,吴佳健,曾夷,胡君,李敏,刘鸿颖,李金芬..茯苓酸调控Sirt1/PGC-1α通路改善妊娠高血压大鼠肾损伤的机制[J].中国药房,2026,37(2):186-191,6.基金项目
江西省自然科学基金项目(No.2024BAC205023) （No.2024BAC205023）
江西省卫生健康委科技计划项目(No.202120014) （No.202120014）
