作物学报2026，Vol.52Issue(2)：644-652,9.DOI:10.3724/SP.J.1006.2026.55045
花生机械脱壳损伤相关农艺指标筛选与QTL定位
Screening and QTL mapping for mechanical shelling damage related traits in peanut
摘要
Abstract
To further identify agronomic traits influencing mechanical shelling quality in peanut and explore their genetic basis,this study evaluated 63 advanced-generation lines and focused on three pod-or kernel-related traits:shelling percentage,pod crushing force,and kernel crushing force.Correlation analysis revealed that only kernel crushing force was significantly associ-ated with the damaged kernel rate.Two lines(D9 and E10)with low damaged kernel rates(<2.50%)were identified.Additionally,a recombinant inbred line(RIL)population of 181 individuals derived from a cross between cultivars Huayu 36 and 6-13 was used for QTL mapping of the kernel crushing force trait.Phenotypic data were collected from 2020 to 2023 across four locations:Yantai,Weihai,Dongying,and Qingdao.The trait exhibited continuous variation and transgressive segregation in the RIL popula-tion,with a broad-sense heritability of 0.88.Using a previously published high-density genetic map,eight additive QTLs associ-ated with kernel crushing force were identified,explaining 6.04%to 28.30%of the phenotypic variation.Among them,two major QTLs,qKCF7 and qKCF16.1,were stably expressed across multiple environments,with favorable alleles derived from Huayu 36.In addition,12 pairs of epistatic QTLs involving 24 SNP intervals were detected,explaining 1.55%to 4.01%of the phenotypic variation.These findings provide valuable genetic targets and germplasm resources for the future genetic improvement of traits related to mechanical shelling quality in peanut.关键词
花生/机械脱壳/籽仁破损压力/数量性状位点/定位Key words
peanut/mechanical shelling/kernel crushing force/quantitative trait locus/mapping引用本文复制引用
张胜忠,李国卫,戈立江,王菲菲,胡晓辉,苗华荣,李燕,钟文,陈静..花生机械脱壳损伤相关农艺指标筛选与QTL定位[J].作物学报,2026,52(2):644-652,9.基金项目
本研究由国家重点研发计划项目(2023YFD1202800),山东省自然科学基金项目(ZR2022MC045)和山东省农业科学院重大科技成果培育工程项目(CXGC2025E02)资助.This study was supported by the National Key Research and Development Program of China(2023YFD1202800),the Natural Science Foundation of Shandong Province(ZR2022MC045),and the Major Scientific and Technological Achievements Cultivation Program of Shandong Academy of Agricultural Sciences(CXGC2025E02). （2023YFD1202800）
