中山大学学报（医学科学版）2026，Vol.47Issue(1)：11-16,6.DOI:10.11714/jsysu.med.YX20250099
应用cytb序列鉴定野鼠粪便中的广州管圆线虫感染
Identification of Angiostrongylus cantonensis in Wild Rodent Feces via cytb Sequences
摘要
Abstract
[Objective]Several molecular markers were evaluated for their reliability in detecting Angiostrongylus cantonensis in wild rodent feces and for determining the infection status of these rodents.[Methods]A total of 128 wild rodents were captured in Huashan Town,Huadu district,Guangzhou.Their fecal samples were collected and total DNA was extracted with the FastDNA SPIN Kit for Soil.PCR amplification was performed to obtain cytb,cox I and ITS2 gene fragments,followed by Sanger sequencing and phylogenetical analysis.[Results]Among all 128 fecal samples,only 5 had clean PCR bands and unambiguous cytb sequences suitable for reliable species identification.The infection rate of A.cantonensis was 7.58%(5/66)in Rattus norvegicus,whereas no cytb fragments were detected in Rattus tanezumi,Rattus losea,Suncus murinus and Bandicota indica.These data indicate that R.norvegicus is a highly suitable host for A.cantonensis,and the cytb gene serves as an effective molecular marker for detecting A.cantonensis in wild rodent feces.[Conclusion]The cytb is an effective molecular marker for detecting A.cantonensis in wild rodent feces,enabling long-term surveillance of its prevalence and potential public health threat.关键词
广州管圆线虫/cytb/野鼠/粪便/调查Key words
Angiostrongylus cantonensis/cytb/wild rodent/feces/investigation分类
农业科技引用本文复制引用
吕进微,林玉,冯耀宇,肖立华,元冬娟..应用cytb序列鉴定野鼠粪便中的广州管圆线虫感染[J].中山大学学报（医学科学版）,2026,47(1):11-16,6.基金项目
广东省基础与应用基础研究基金(2023A1515011964,2024A1515011658) （2023A1515011964,2024A1515011658）
