中西医结合慢性病杂志2026，Vol.3Issue(1)：9-14,6.DOI:10.26950/j.issn.2097-5031.2026.01.002
基于NF-κB/I-κB通路探讨坤灵丸改善DOR大鼠卵巢功能机制
Mechanism of Kunling Pill in Improving Ovarian Function in DOR Rats Via the NF-κB/I-κB Pathway
摘要
Abstract
Objective To investigate the mechanism by which Kunling Pill improves cyclophosphamide(CTX)-induced diminished ovarian reserve(DOR)in rats by regulating the nuclear factor kappa B(NF-κB)/inhibitor of nuclear factor kappa B(I-κB)signaling pathway.Methods 45 Sprague Dawley(SD)female rats with normal estrous cycles were randomly divided into a normal control group,a model group,a positive drug group(estradiol valerate),a low-dose Kunling Pill group(0.5 g/kg),and a high-dose Kunling Pill group(1 g/kg),with 9 rats in each group.Except for the normal control group,all groups were established with a single injection of CTX(90 mg/kg)to establish a rat DOR model.After 28 days of continuous oral administration,serum hormones estradiol(E2),anti-Müllerian hormone(AMH),follicle stimulating hormone(FSH),inflammatory factors interleukin-1 beta(IL-1 β),interleukin-4(IL-4),interleukin-6(IL-6),and ovarian tissue NF-κB/IκB protein expression were detected.Results As a result,Kunling Pill can regulate the hormone levels in rats.Compared with the model group,the low-dose Kunling Pill group and high-dose Kunling Pill group showed significant increases in serum AMH and E2,while FSH showed no significant changes.Kunling Pill reduces the accumulation of IL-1β and IL-6 in the serum of DOR rats,and lowers the levels of NF-κB and I-κB to alleviate inflammatory reactions.Conclusion Kunling Pill can improve ovarian reserve function.The mechanism may be mediated by inhibiting the NF-κB/I-κ B pathway to alleviate inflammation induced ovarian damage and improve rat ovarian function.关键词
坤灵丸/卵巢储备功能/NF-κB/I-κB/炎症Key words
Kunling Pill/ovarian reserve/NF-κB/I-κB/inflammation分类
医药卫生引用本文复制引用
王海燕,卫博文,曹丹..基于NF-κB/I-κB通路探讨坤灵丸改善DOR大鼠卵巢功能机制[J].中西医结合慢性病杂志,2026,3(1):9-14,6.基金项目
辽宁省自然科学基金面上项目(2023-MS-344) （2023-MS-344）
大连市医学科学研究计划项目(22Z11020) （22Z11020）
