植物遗传资源学报2026，Vol.27Issue(1)：87-99,13.DOI:10.13430/j.cnki.jpgr.20250731001
我国烟豆种质资源搜集取样策略研究
Sampling Strategies for Germplasm Collection of Glycine tabacina(Labill.)Benth
摘要
Abstract
Glycine tabacina(Labill.)Benth.,a perennial species of the subgenus Glycine,is endemic to Fujian province,China,and was listed as a National Grade Ⅱ Key Protected Wild Plant.Rational population sampling to maximize the preservation of genetic diversity is crucial for germplasm collection of this species.In this study,species-specific SSR markers were employed to analyze both inter-population genetic structures across 13 natural populations and intra-population genetic structures in a single-population.The results show that geographically there were extremely low gene differentiation(Fs=0.056),high expected heterozygosity(He=0.741),high polymorphism information content(PIC=0.70),and substantial gene flow(Nm=4.445);intra-population variation(Hs accounting for 78.0％of the total variation)exceeded inter-population differentiation(Dst accounting for 22.0％of the total variation).The results of this study clearly support that:although G.tabacina is a self-pollinating plant,its geospatial population genetic variation characteristic resembles that of outcrossing plants,which may be attributed to the species' perennial nature facilitating high genetic heterogeneity and extensive long-distance dispersal enabling high geographical gene flow.The fine-scale spatial genetic structure analysis within the single population(Longhai)with higher-level disturbance revealed that the size range of spatial genetic patches among plants was about 5.0 m.When the sampling size was 20-25 plants,95％of the population's genetic diversity was captured.Based on the findings of this study,we propose the following strategies for collecting G.tabacina germplasm resources:at the population level,at least 20-25 individual plants should be sampled within a population,with an interval of at least 9-15 m between sampled plants.At the geographical spatial level of populations,since G.tabacina in China is only distributed in a narrow coastal area of Fujian province,it is recommended to collect as many populations as possible.关键词
烟豆/遗传结构/SSR分析/基因流/取样策略Key words
Glycine tabacina/genetic structure/SSR analysis/gene flow/sampling strategy引用本文复制引用
王克晶,时英喆,王浩辰,李向华..我国烟豆种质资源搜集取样策略研究[J].植物遗传资源学报,2026,27(1):87-99,13.基金项目
科技部国家重点研发计划(2021YFD1200103)National Key R&D Program of the Ministry of Science and Technology(2021YFD1200103) （2021YFD1200103）
