中国比较医学杂志2025，Vol.35Issue(12)：31-39,9.DOI:10.3969/j.issn.1671-7856.2025.12.003
玉朗伞查尔酮对血管紧张素Ⅱ诱导的H9c2心肌细胞损伤的影响及机制研究
Effect and mechanism of Yulangsan Chalcone on angiotensin Ⅱ-induced H9c2 myocardial injury
摘要
Abstract
Objective To investigate the protective effect of Yulangsan Chalcone(YLSC)on H9c2 cardiomyocyte injury induced by angiotensin Ⅱ(Ang Ⅱ)and its possible mechanism.Methods H9c2 cardiomyocytes were divided into blank control,model,and low-,medium-,and high-dose YLSC groups.After intervention with YLSC,the cells were induced with Ang Ⅱ(1 μmol/L)to form an injury model.Cell viability was detected by cell counting kit-8 assay,autophagosomes were detected by monodansylcadaverine staining,reactive oxygen species were detected using the dichlorodihydrofluorescein diacetate fluorescence probe method,apoptosis was detected by Hoechst 33342 staining,and the ATP content was detected by enzyme-linked immunosorbent assay.Bcl-2 interacting protein 3(Bnip-3)and autophagy-related protein 5(Atg5)gene levels were detected by reverse transcription-polymerase chain reaction,and expression levels of the autophagy marker proteins p62,Beclin1,and microtubule-associated protein light chain 3(LC3)Ⅱ/Ⅰ were detected by Western blot.Results YLSC alleviated Ang Ⅱ-induced H9c2 cardiomyocyte injury by enhancing cardiomyocyte viability(P＜0.05 or P＜0.01),promoting the formation of autophagosomes,and reducing the generation of reactive oxygen species(P＜0.01).YLSC also reduced cell apoptosis(P＜0.01),increased ATP levels in cells(P＜0.05 or P＜0.01),promoted the gene expression of Bnip-3 and Atg5(P＜0.05 or P＜0.01),and reduced the expression of p62 and increased the expression of Beclin1 and LC3Ⅱ/LC3I(P＜0.05 or P＜0.01).Conclusions YLSC can effectively inhibit Ang Ⅱ-induced H9c2 cardiomyocyte injury and apoptosis,and its mechanism may be related to the initiation of myocardial autophagy,activation of autophagy-related factors,and clearance of apoptotic cells.关键词
玉朗伞查尔酮/心肌细胞损伤/自噬/H9c2心肌细胞Key words
Yulangsan Chalcone/cardiomyocyte injury/autophagy/H9c2 cardiomyocytes分类
医药卫生引用本文复制引用
梁杏梅,农慧亮,廖乃英,刘安韬,蒙泔竹,黄仁彬,张婷,傅鹏..玉朗伞查尔酮对血管紧张素Ⅱ诱导的H9c2心肌细胞损伤的影响及机制研究[J].中国比较医学杂志,2025,35(12):31-39,9.基金项目
广西科技计划项目(桂科AB25069015,2023GXNSFBA026148) （桂科AB25069015,2023GXNSFBA026148）
广西壮族自治区中医药管理局自筹经费科研课题(GXZYA20230245) （GXZYA20230245）
广西卫生职业技术学院校级科研型创新团队建设(壮瑶药研发创新团队、"桂派"中药特色炮制技术与药膳预制品开发). （壮瑶药研发创新团队、"桂派"中药特色炮制技术与药膳预制品开发）
