中国比较医学杂志2025，Vol.35Issue(12)：40-51,12.DOI:10.3969/j.issn.1671-7856.2025.12.004
干扰骨桥蛋白表达对肝癌细胞Warburg效应的影响及机制
Effect and mechanism of osteopontin interference on the Warburg effect in hepatoma cells
摘要
Abstract
Objective To investigate the effect of osteopontin(OPN)interference on the Warburg effect in hepatoma cells and to explore the related molecular mechanism.Methods Small interfering(si)RNA targeting OPN(si-OPN)and negative Control siRNA(si-NC)were transfected into HepG2 and SK-HEP-1 cells.The glucose-uptake capacity of hepatoma cells was detected using a fluorescent probe(2-NBDG),and lactate production in HepG2 and SK-HEP-1 cells was evaluated using a lactate-detection kit.Expression levels of the glycolysis-related genesand proteins glucose transporter type 1(GLUT1),hexokinase 2(HK2),and lactate dehydrogenase A(LDHA)were determined by quantitative reverse transcription-polymerase chain reaction and Western blot.We then silenced the expression of galectin-3-binding protein(LGALS3BP)in HepG2 and SK-HEP-1 cells,and overexpressed LGALS3BP in OPN-silenced HepG2 and SK-HEP-1 cells,and evaluated the expression levels of LGALS3BP,GLUT1,HK2,and LDHA,and the glucose-uptake capacity and lactate production in the above cells.Results si-OPN significantly reduced glucose uptake and lactate production in HepG2 and SK-HEP-1 cells compared with the Control and si-NC groups,and significantly reduced GLUT1,HK2,and LDHA gene and protein expression levels(P＜0.05).si-OPN reduced LGALS3BP protein expression in HepG2 and SK-HEP-1 cells,and silencing LGALS3BP reduced glucose uptake,lactate generation,and the expression levels of GLUT1,HK2,and LDHA(P＜0.05).Overexpression of LGALS3BP rescued the suppression of the above glycolytic metabolism-related indicators(P＜0.05).These result suggest that OPN stimulated glycolytic metabolism in hepatocellular carcinoma cells via LGALS3BP.Conclusions OPN can affect the expression of glycolysis-related genes via LGALS3BP,thereby regulating the Warburg effect in hepatoma cells.关键词
肝细胞癌/骨桥蛋白/Warburg效应/LGALS3BPKey words
hepatocellular carcinoma/osteopontin/Warburg effect/LGALS3BP分类
医药卫生引用本文复制引用
林婷,安日问,赵方新,曲蕾,樊文轩,武建强,张烜..干扰骨桥蛋白表达对肝癌细胞Warburg效应的影响及机制[J].中国比较医学杂志,2025,35(12):40-51,12.基金项目
内蒙古自治区自然科学基金项目(2022MS08009) （2022MS08009）
内蒙古医科大学面上项目(YKD2023MS046) （YKD2023MS046）
内蒙古医科大学博士启动基金项目(YKD2023BSQD006) （YKD2023BSQD006）
内蒙古医科大学"致远"人才项目(DC2400001593) （DC2400001593）
大学生创新创业训练计划项目(创客培育)(101322024079). （创客培育）
