中兽医医药杂志2026，Vol.45Issue(1)：1-6,封2,7.DOI:10.13823/j.cnki.jtcvm.2025.081
基于代谢组学探讨单宁酸对金黄色葡萄球菌的抑菌机制
Exploring the antibacterial mechanism of tannic acid against Staphylococcus aureus based on metabolomics
摘要
Abstract
To investigate the antibacterial mechanism of tannic acid against Staphylococcus aureus,the Minimum Inhibitory Concentration(MIC)and Minimum Bactericidal Concentration(MBC)of tannic acid against methicillin-resistant Staphylococcus aureus(MRSA)USA300 were determined using the broth microdilution method.Subsequently,liquid chromatography-mass spectrometry(LC-MS)was employed to analyze the effects of tannic acid at 1/2 MIC on the metabolites of MRSA USA300.Finally,the screened metabolites were subjected to Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis to further explore the antibacterial mechanism of tannic acid against MRSA.The results showed that the MIC of tannic acid against MRSA USA300 was 0.625 mg/mL,and the MBC was 1.250 mg/mL.Under the action of tannic acid at 1/2 MIC(0.312 5 mg/mL),7 differentially expressed metabolites were identified(P<0.05,FC≥1.2 or≤1/1.2).Among them,the expression of PG(35:3)and 2,6-Di-tert-butyl-4-nitrophenol were significantly upregulated(P<0.05),while the expression of 13-docosenamide(Z),PC(18:0/18:2),2-hydroxy-dAMP,D-3-phosphoglyceric acid,and PG(23:0)were significantly downregulated(P<0.05).KEGG pathway enrichment analysis revealed that these differential metabolites were enriched in glycerophospholipid metabolism pathway,glycolysis/gluconeogenesis,glycine,serine and threonine metabolism,and other pathways.Furthermore,based on the KEGG information of differential metabolites,a regulatory relationship network diagram between metabolites and pathways was constructed,which showed that D-3-phosphoglyceric acid occupied a central position and might be the key metabolite for tannic acid to inhibit MRSA.The results of this experiment indicated that tannic acid exhibited in vitro antibacterial activity against MRSA,and its mechanism might be related to affecting glycerophospholipid metabolism,glycolysis/gluconeogenesis,pentose phosphate pathway,glycine,serine and threonine metabolism,as well as cysteine and methionine metabolism pathways.D-3-phosphoglyceric acid might be the key metabolite for tannic acid to inhibit MRSA.This study can provide insights for further elaborating the antibacterial mechanism of tannic acid.关键词
单宁酸/金黄色葡萄球菌/代谢组学/抑菌机制Key words
tannic acid/Staphylococcus aureus/metabolomics/antibacterial mechanism分类
农业科技引用本文复制引用
王爽,彭玮,杨苗,伍忠旭,周永辉..基于代谢组学探讨单宁酸对金黄色葡萄球菌的抑菌机制[J].中兽医医药杂志,2026,45(1):1-6,封2,7.基金项目
国家自然科学基金项目(82560726) （82560726）
贵州省科技计划项目(黔科合基础-ZK[2024]一般349) （黔科合基础-ZK[2024]一般349）
贵州中医药大学博士启动基金项目(贵中医博士启动[2020]22号) （贵中医博士启动[2020]22号）
