中兽医医药杂志2026，Vol.45Issue(1)：7-13,7.DOI:10.13823/j.cnki.jtcvm.2025.083
鹿茸菇多糖的提取工艺优化及其体外抗氧化作用研究
Optimization of extraction process for polysaccharides from Lyophyllum decastes by orthogonal test and their antioxidant activity in vitro
摘要
Abstract
For the efficient preparation of Lyophyllum decastes polysaccharides(LDPs),this study systematically compared the effects of five extraction methods—hot water extraction(HWE),enzyme-assisted aqueous extraction with alcohol precipitation(EAAE),microwave-assisted water extraction(MAE),ultrasound-assisted water extraction(UAE),and freeze-thaw-assisted water extraction(FTE)—on the extraction yield and polysaccharide content.The results showed the following respective yields and polysaccharide contents:HWE:(4.63±0.40)%and(41.87±1.39)%;EAAE:(19.38±1.90)%and(77.03±1.76)%;MAE:(7.32±0.47)%and(44.78±0.76)%;UAE:(8.57±0.48)%and(42.57±1.36)%;FTE:(11.46±0.50)%and(54.59±2.22)%.Among these,EAAE demonstrated the best performance and was therefore selected for LDPs extraction.Subsequently,based on single-factor experiments,the extraction process was optimized using an orthogonal array design focusing on four key parameters:extraction cycles,enzymatic hydrolysis time,enzyme concentration,and solid-liquid ratio.The optimal conditions were determined as follows:3 extraction cycles,150 min enzymatic hydrolysis time,2.5%enzyme concentration,and a solid-liquid ratio of 1∶20(g∶mL).Under these optimized conditions,triple validation experiments confirmed the process's stability and reliability,showing polysaccharide contents of 83.19%,82.08%,and 81.96%,and extraction yields of 20.18%,21.34%,and 20.96%,respectively.Furthermore,in vitro antioxidant activity assays revealed that the prepared LDPs exhibited potent scavenging abilities against both DPPH·and ABTS+·in a dose-dependent manner.At a concentration of 4.0 mg/mL,the scavenging rates reached 80.17%for DPPH·and 88.42%for ABTS+·.In conclusion,this study successfully established an efficient extraction process for LDPs and confirmed their significant antioxidant potential,providing a crucial foundation for the development and application of Lyophyllum decastes in functional foods.关键词
鹿茸菇多糖/正交试验/抗氧化活性Key words
Lyophyllum decastes polysaccharides/orthogonal test/antioxidant activity分类
农业科技引用本文复制引用
李艳华,周淑棉,何颖,胡庭俊..鹿茸菇多糖的提取工艺优化及其体外抗氧化作用研究[J].中兽医医药杂志,2026,45(1):7-13,7.基金项目
国家自然科学基金资助项目(32560871) （32560871）
广西研究生教育创新计划项目(YCSW2025084) （YCSW2025084）
南宁市科学研究与技术开发计划项目(20232039) （20232039）
