植物研究2026，Vol.46Issue(1)：91-100,10.DOI:10.7525/j.issn.1673-5102.2026.01.008
复幼措施对水曲柳生长与组培再生的影响
Impact of Rejuvenation Measures on Growth and Tissue Culture Regeneration of Fraxinus mandshurica
摘要
Abstract
As an essential method for clonal propagation of the valuable timber species Fraxinus mandshurica,tissue culture is hindered by physiological aging of explants due to age-related effects,which significantly reduces tissue culture efficiency and severely restricts the rapid clonal propagation and widespread application of superior F.mandshurica varieties.Currently,there are no reports on overcoming the age effect through rejuvenation to improve tissue culture efficiency in forest trees.This study investigated the effects of different rejuvenation treatments on growth performance and tissue culture regeneration of 4-year-old F.mandshurica plants.The results showed that after trunk cutting+shading treatment,the height and diameter of new shoots decreased by 37.50%and 42.90%,respectively,while the number of new shoots and stem segment explants increased by 2.6-fold and 2.8-fold,which significantly enhanced tissue culture regeneration capacity.The initiation rate of stem segment explants,new shoot differentiation rate,and propagation coefficient of the shoots under treatment all increased compared to the untreated control(CK).The highest initiation rate reached 82.42%,which was 3.26 times as high as that of the CK(25.30%).The differentiation rate increased from 9.90%to 61.11%.The propagation coefficient increased from 0 to 6.00.Further investigation into the mechanism by which rejuvenation measures overcome the age effect was conducted through the analysis of physiological and biochemical indicators and related gene expression changes.It was found that after rejuvenation treatment,sucrose and soluble sugar content significantly decreased by 23.17%and 46.24%,while the content of auxin(IAA)and cytokinin(CTK)significantly increased by 70.01%and 57.22%,respectively.Based on the trend of miR156A/C gene expression downregulating with plant maturation,transcriptional analysis of the miR156-SPL-AP2/ERFs-ASA1 gene pathway further revealed that after trunk cutting+shading treatment,the expression levels of rejuvenation and bud regeneration-related genes FmmiR156A,FmmiR156C,and FmASA1 increased by 10.01,13.53,and 12.58 times compared to CK,while the expression levels of FmSPL2 and FmSPL9 decreased by 93.05%and 88.30%compared to CK.These findings indicated that the above indicators may be key factors promoting rejuvenation in F.mandshurica,thereby improving the tissue culture regeneration efficiency of explants.This study provided practical methods for addressing the age effect and improving tissue culture propagation capacity in F.mandshurica and other forest trees species,offering technical support for the large-scale propagation and promotion of superior varieties and new cultivars of F.mandshurica.关键词
水曲柳/组织培养/年龄效应/复幼处理/生理生化指标/基因表达Key words
Fraxinus mandshurica/tissue culture/age effect/rejuvenation treatment/physiological and biochemical indicators/gene expression分类
农业科技引用本文复制引用
熊成明,王巧欣,何利明,刘建飞,彭广州,胡安红,李新宇,詹亚光..复幼措施对水曲柳生长与组培再生的影响[J].植物研究,2026,46(1):91-100,10.基金项目
国家重点研发计划项目(2021YFD2200303). （2021YFD2200303）
