植物研究2026，Vol.46Issue(1)：101-110,10.DOI:10.7525/j.issn.1673-5102.2026.01.009
半夏试管块茎延缓生长保存技术
Conservation Technology of In Vitro Tubers of Pinellia ternata by Delayed Growth
摘要
Abstract
To establish a technology system for the delayed growth preservation of Pinellia ternata tubers in vitro,this study valuated different sucrose mass concentrations,the ratio of sucrose to mannitol,and low-temperature methods to preserve the in vitro tubers.After 360 days,the tubers were allowed to resume growth,and the growth indicators were observed and statistically analyzed.Starch grain dynamics in the tubers were examined using paraffin sectioning technique.The genetic stability of regenerated plants after preservation was assessed using ISSR-PCR and RAPD molecular markers.Mature tubers were inoculated on fresh 1/2MS+30 g⋅L-1 sucrose+60 g⋅L-1 mannitol medium and stored at 4℃ for 360 days,the sprouting rate of tubers after recovery growth was as high as 96.67％,and the plants grew well with the best preservation effect.Tubers cultured on medium containing 90 g⋅L-1 sucrose,showed reduced growth despite a sprouting rate of 90.00％,likely due to the excessively high osmotic pressure.When mature tubers were stored in the old medium of 1/2MS+30 g⋅L-1 sucrose at 4℃ for 360 days,the sprouting rate of tubers after recovery growth was the lowest,only 36.70％.Histological studies showed that in the dead tubers after preservation,there were a few leaf primordia,almost no starch grains in the cells,and no mucilage cells.Both the high sprouting rate and low sprouting rate treatments had a large amount of starch grains stored in the tubers after recovery growth,but the treatment with high survival rate had more layers of leaf primordia in the stem tips and fewer mucilage cells,and the plants grew more vigorously.A total of 1 140 bands were amplified using molecular marker techniques,and no variant bands were detected,indicating genetic stability of regenerated plants after delayed growth preservation.This study established a simple and efficient technique for the delayed growth preservation of P.ternata in vitro tubers,providing a feasible approach for short-term preservation of P.ternata germplasm.关键词
半夏/试管块茎/延缓生长/再生植株/淀粉粒分布/遗传稳定性Key words
Pinellia ternata/in vitro tuber/delayed growth preservation/regenerated plants/starch granule distribution/genetic stability分类
农业科技引用本文复制引用
张延红,张亚萍,王琳佳,何春雨,郭清毅..半夏试管块茎延缓生长保存技术[J].植物研究,2026,46(1):101-110,10.基金项目
甘肃省高等学校产业支撑计划项目(2020C-09) （2020C-09）
甘肃省联合基金项目(24JRRA877) （24JRRA877）
甘肃省重点研发计划项目(24YFNA007) （24YFNA007）
甘肃中医药大学成果转化培育项目(2023CGZH-18) （2023CGZH-18）
甘肃中医药大学教学改革项目(ZHXM-2021-17). （ZHXM-2021-17）
