植物研究2026，Vol.46Issue(1)：120-130,11.DOI:10.7525/j.issn.1673-5102.2026.01.011
灵武长枣果实发育过程中阿拉伯半乳糖蛋白胶体金免疫分布特征
The Colloidal Gold Immune Distribution Characteristics of Arabinogalactan Proteins during Fruit Development of Ziziphus jujuba'Lingwu Changzao'
摘要
Abstract
Arabinogalactan proteins(AGPs)play an important role in fruit ripening and softening.In order to explore the distribution characteristics and dynamic changes of AGPs in fruits of Ziziphus jujuba'Lingwu Changzao'during development,the monoclonal antibody JIM13 and colloidal gold immunoelectron microscopy technology were used to analyze the ultrastructural characteristics and the immunological cell distribution of AGPs of Ziziphus jujuba'Lingwu Changzao'fruits at four different developmental stages.The results showed that after labeled with 10 nm colloidal gold particles,there were a large number of gold particles distributed in the cell interior and cell walls of parenchymal cells in the phloem and far away from the vascular bundles,and there were also gold particles distributed in the vacuoles,plastids and other organelles during the early stage of fruit enlargement.During the rapid enlargement period,there were a large number of gold particles distributed in the cell walls and cell interior of sieve tube molecules and parenchymal cells far away from the vascular bundles.During the coloring period,there were a few gold particles in the cell walls and cell interior of sieve tubes,companion cells and parenchymal cells in phloem of vascular bundles,as well as parenchymal cells far away from the vascular bundles.During the maturation period,in the companion cells in phloem of vascular bundles,as well as parenchymal cells far away from vascular bundles,there were a small number of gold particles on the cell walls and close to the plasma membrane,and a few gold particles inside the cell wall,and on the vesicles,mitochondria,plastids and large central vacuoles.The results suggested that AGPs were mainly distributed on the cell wall,near the plasma membrane,inside the cell and the organelles,and there were some differences in distribution of AGPs in fruits at different developmental stages:more AGPs distributed in the cell wall and inside the cell in the early bulking period and the rapid enlargement period,and a small number of AGPs distributed in the cell wall and inside the cell in the coloring and ripening periods.The results provided cytological basis for revealing the effect of AGPs on fruit quality.关键词
灵武长枣/果实/阿拉伯半乳糖蛋白/胶体金免疫电镜Key words
Ziziphus jujuba'Lingwu Changzao'/fruit/arabinogalactan proteins/colloidal gold immunoelectron microscopy分类
农业科技引用本文复制引用
马小玲,章英才,王静,郑国琦..灵武长枣果实发育过程中阿拉伯半乳糖蛋白胶体金免疫分布特征[J].植物研究,2026,46(1):120-130,11.基金项目
宁夏自然科学基金项目(2022AAC03087). （2022AAC03087）
