作物学报2026，Vol.52Issue(2)：349-362,14.DOI:10.3724/SP.J.1006.2026.55035
Bna-miR1040-EIF3A模块调控油菜开花时间的功能研究
Functional analysis of the Bna-miR1040-EIF3A module in regulating flowering time in rapeseed(Brassica napus)
摘要
Abstract
Flowering time is a critical developmental trait in crops that significantly influences yield.MicroRNAs(miRNAs),a class of small endogenous RNAs processed from MIR gene transcripts,regulate gene expression by mediating transcript cleavage.In this study,we identified a novel miRNA—novel-Bna-miR1040—in rapeseed(Brassica napus),whose biological function had not been previously characterized.Transgenic rapeseed plants overexpressing Bna-miR1040 exhibited delayed flowering com-pared to wild-type plants.Degradome sequencing identified BnaEIF3A as a potential target of Bna-miR1040.This interaction was validated by 5′RACE and transient expression assays in tobacco leaves,which confirmed that Bna-miR1040 cleaves BnaEIF3A,significantly reducing its transcript levels.Evolutionary analysis showed that BnaEIF3A is highly conserved across diverse plant species.Subcellular localization analysis revealed that the BnaEIF3A protein is exclusively localized in the nucleus.To further investigate its function,we generated BnaEIF3A loss-of-function mutants using CRISPR/Cas9 genome editing.These mutants also exhibited delayed flowering phenotypes compared to wild-type controls.Together,our findings demonstrate that Bna-miR1040 regulates flowering time in rapeseed by suppressing BnaEIF3A expression,providing novel insights for molecular breeding strategies aimed at optimizing flowering time in rapeseed.关键词
甘蓝型油菜/miRNA/Bna-miR1040/EIF3A/开花时间Key words
Brassica napus/miRNA/Bna-miR1040/EIF3A/flowering time引用本文复制引用
马毅娜,沈金雄,吴晓明玉,李藕琪,王圆,陈丽,张盈川,赵伦,文静,傅廷栋..Bna-miR1040-EIF3A模块调控油菜开花时间的功能研究[J].作物学报,2026,52(2):349-362,14.基金项目
本研究由国家自然科学基金项目(31930032)和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-12)资助.This study was supported by the National Natural Science Foundation of China project(31930032)and the China Agriculture Research Sys-tem of MOF and MARA(CARS-12). （31930032）
