中国中西医结合杂志2026，Vol.46Issue(1)：59-66,8.DOI:10.7661/j.cjim.20251111.215
解毒生肌膏调控Ang/Tie2/VEGF信号通路促进糖尿病溃疡血管生成
Jiedu Shengji Plaster Promotes Angiogenesis of Diabetic Ulcers via Regulating Ang/Tie2/VEGF Signalling Pathway
摘要
Abstract
Objective To explore the mechanism by which Jiedu Shengji Plaster promotes diabetic ulcer repair based on the signalling pathways of vascular endothelial growth factor(VEGF),angiopoietin(Ang),and tyrosine kinase(Tie2).Methods Forty-eight SD rats were randomly divided into the normal control(n=12)and model groups(n=36),induced via high-fat diet combined with intraperitoneal injection of streptozotocin to create full-thickness skin defects on the back,and the rat model of diabetes ulcer was established.The model rat were randomly divided into model group,detoxification group and sulfonamide group,12 per group.After disinfection,the normal and model groups received Vaseline cream,whereas the detoxification and sulfonamide groups were treated with Jiedu Shengji Plaster and sulfonamide silver,respectively,with daily dressing changes.Wound healing rates were calculated from images obtained at days 0,3,7,and 14.After 14 days,samples were collected for histological and molecular analyses.HE staining was used for tissue inflammation,granulation tissue,and vascular conditions.Collagen volume was detected by collagen staining.Immunohistochemistry was used to detect Ang1,Ang2,and Tie2 expressions in wounds,and Western Blot for VEGF,Ang1,Ang2,and Tie2 protein levels in wound tissues.Results After 7 and 14 days of wound treatment,compared with normal group,the wound healing rate decreased(P＜0.01),inflammation increased,collagen decreased(P＜0.01),VEGF expression decreased(P＜0.01),Ang1 and Tie2 protein expression around blood vessels and wound tissue protein expression level decreased(P＜0.01),and Ang2 protein expression around blood vessels and wound tissue expression level increased in the model group(P＜0.01).Compared with the model group,the detoxification group showed significantly increased wound healing rate(P＜0.01),decreased inflammation,increased neovascularization,increased collagen(P＜0.01),increased VEGF expression(P＜0.01),increased Ang1 and Tie2 protein expression around blood vessels and wound tissue protein expression level(P＜0.01),and decreased Ang2 expression in blood vessels and wound tissue expression level(P＜0.01).The sulfonamide group showed a reversal in wound healing rate,inflammatory infiltration,Ang1,Tie2,and Ang2,however.However,all were weaker than the detoxification group(P＜0.01,P＜0.05).Conclusion Jiedu Shengji Plaster promotes angiogenesis and accelerates the healing of diabetic ulcers simultaneously with early anti-inflammatory effects,and the mechanism may be related to Ang/Tie2/VEGF signalling pathway regulation.关键词
解毒生肌膏/血管紧张素/血管紧张素受体酪氨酸激酶2/血管内皮细胞生长因子信号通路/糖尿病溃疡/血管生成/中药复方Key words
Jiedu Shengji Plaster/Ang/Tie2/VEGF signalling pathway/diabetic ulcer/angiogenesis/Chinese herbal compound引用本文复制引用
廖亮英,丁雅容,黄新灵,刘光余,王栩冰,陈丽,周忠志..解毒生肌膏调控Ang/Tie2/VEGF信号通路促进糖尿病溃疡血管生成[J].中国中西医结合杂志,2026,46(1):59-66,8.基金项目
湖南省临床医疗技术创新引导项目(No.2021SK51401) （No.2021SK51401）
湖南中医药大学省级学科建设项目(中西医结合)(No.湘教通[2022]351) （中西医结合）
国家自然科学基金资助项目(No.82305256) （No.82305256）
湖南省中医药科研课题(No.B2023097) （No.B2023097）
