中国中西医结合杂志2026，Vol.46Issue(1)：67-74,8.DOI:10.7661/j.cjim.20251122.226
荣筋拈痛方抑制PARP1延缓软骨细胞衰老治疗膝骨关节炎
Rongjin Niantong Formula Alleviates Knee Osteoarthritis by Attenuating Chondrocyte Senescence via Inhibiting PARP1
摘要
Abstract
Objective To investigate the mechanism of Rongjin Niantong Formula(RJNTF)in treating knee osteoarthritis(KOA)via in delaying chondrocyte senescence through the modulation of Poly(ADP-ribose)polymerase 1(PARP1).Methods This study was divided into two experiments.In experiment one,chondrocytes were divided into the si-NC group(negative control virus),the si-PARP1 group(PARP1 knockdown),the D-galactose(D-gal)+si-NC group(negative control virus+D-gal)and the D-gal+si-PARP1 group(PARP1 knockdown+D-gal).In experiment two,chondrocytes were divided into the normal group(10％blank serum),the model group(10％blank serum+10 g/L D-gal),the treatment group(10％RJNTF drug-containing serum+10 g/L D-gal)and the inhibitor group(10％blank serum+10 g/L D-gal+10 μmol/L PJ34).Senescence was assessed by senescence-associated β-galactosidase(SA-β-gal staining),and cell proliferation was evaluated by the EdU assay.NAD+levels and the NAD+/nicotinamide adenine dinucleotide(NADH)ratio were measured.Interleukin-6(IL-6),high mobility group box 1(HMGB1),tumor necrosis factor-alpha(TNF-α)were quantified by ELISA.The mRNA and protein expression of Poly ADP-ribose 1(PARP1),multiple tumor suppressor 1(p16)and matrix metallopeptidase 13(MMP-13)were detected by RT-qPCR and Western Blot,respectively.Results Results of experiment 1:Compared with the si-NC group,the positive rate of SA-β-gal,the expressions of IL-6,HMGB1,TNF-α,and the mRNA and protein expressions of PARP1,p16,and MMP-13 in the D-gal+si-NC group increased(P＜0.01).The content of NAD+,the ratio of NAD+/NADH,and the positive rate of EdU decreased(P＜0.05,P＜0.01).Compared with the D-gal+si-NC group,the positive rate of SA-β-gal,the expressions of IL-6,HMGB1,TNF-α,and the mRNA and protein expressions of PARP1,p16,and MMP-13 in the D-gal+si-PARP1 group decreased(P＜0.05,P＜0.01).The content of NAD+,the ratio of NAD+/NADH,and the positive rate of EdU increased(P＜0.05,P＜0.01).Results of experiment 2:Compared with the model group,the positive rate of SA-β-gal,the expressions of IL-6,HMGB1,and TNF-α in the treatment group and the inhibitor group decreased(P＜0.05,P＜0.01),while the content of NAD+,the ratio of NAD+/NADH,and the positive rate of EdU increased(P＜0.05,P＜0.01).The mRNA expressions of PARP1,p16,MMP-13 in the treatment group and p16,MMP-13 in the inhibitor group decreased(P＜0.05),and the protein expressions of PARP1,PAR,p16,MMP-13 in the treatment group and PAR,p16,MMP-13 in the inhibitor group decreased(P＜0.01).Conclusions RJNTF ameliorates chondrocyte senescence in KOA by inhibiting PARP1 expression,reducing the positive rate of SA-β-gal in chondrocytes,decreasing the consumption of NAD+in chondrocytes,promoting the proliferation of chondrocytes,down-regulating the expression of IL-6,HMGB1 and TNF-αcytokines and mRNA and protein expression of PARP1,p16 and MMP-13.关键词
荣筋拈痛方/膝骨关节炎/软骨细胞衰老/多聚ADP核糖聚合酶1/中药复方Key words
Rongjin Niantong Formula/knee osteoarthritis/chondrocyte senescence/PARP1/Chinese herbal compound引用本文复制引用
李璐,郑若曦,戴雨婷,王如意,陈楠,陈俊,吴广文..荣筋拈痛方抑制PARP1延缓软骨细胞衰老治疗膝骨关节炎[J].中国中西医结合杂志,2026,46(1):67-74,8.基金项目
国家自然科学基金资助项目(No.82405443,No.82474553) （No.82405443,No.82474553）
