北方园艺Issue(2)：9-18,10.DOI:10.11937/bfyy.20252046
辣椒KNOX基因家族的全基因组鉴定及逆境表达分析
Genome-wide Identification of the KNOX Gene Family in Pepper and Analysis of Their Expression Under Stress Conditions
摘要
Abstract
Taking cultivated pepper(Capsicum annuum L.)as the test material,bioinformatics analysis was used to study the KNOX gene at the pepper genome level,including the physicochemical properties,gene structure,protein conserved domains,systematic evolution,promoter cis acting elements,and tissue and stress expression patterns of KNOX gene family members,in order to provide reference for the function of KNOX gene in pepper growth and development,as well as stress response.The results showed that there were a total of 7 KNOX gene family members in the pepper genome,distributed on 7 chromosomes.The majority encoded acidic proteins,were all hydrophilic proteins,and were all located in the nucleus.Phylogenetic analysis could divide the pepper KNOX family into two subfamilies,Class Ⅰ and Class Ⅱ,and further into five subgroups.Promoter analysis revealed some cis-acting regulatory elements related to growth and development,hormones,and stress responses.Tissue expression analysis showed that the Class Ⅰ genes had obvious tissue specificity and were mainly highly expressed in roots,stems,and leaves;the Class Ⅱ genes had a more extensive spatiotemporal expression.Under seven abiotic stress treatments,the expression levels of some genes changed significantly,among which five genes responded to specific stresses.RT-qPCR verified two genes that specifically responded to cold stress,salt stress,and heat stress.关键词
辣椒/全基因组鉴定/KNOX转录因子/逆境胁迫Key words
pepper/whole genome identification/KNOX transcription factor/stress of adversity分类
农业科技引用本文复制引用
陈奕博,李喜娥,白锐琴,张亚星,刘赵帆,李明..辣椒KNOX基因家族的全基因组鉴定及逆境表达分析[J].北方园艺,2026,(2):9-18,10.基金项目
平凉市技术创新引导计划-农业类资助项目(PL-STK-2022A-073) （PL-STK-2022A-073）
平凉市科技重大专项计划-农业类资助项目(PL-STK-2023A-023,PL-STK-2024A-033) （PL-STK-2023A-023,PL-STK-2024A-033）
平凉市农业科学院院列科技创新专项资助项目. （）
