北方园艺Issue(2)：19-26,8.DOI:10.11937/bfyy.20252200
基于梨转录组的SSR分子标记的开发与应用
Development and Application of SSR Molecular Markers Based on the Transcriptome of Pear
摘要
Abstract
Taking the graft union of the'QAUP-1/Qingzhen D1'grafting combination as the test material,methods included transcriptome sequencing,Trinity de novo assembly,Hisat2 sequence alignment,MISA locus searching,and Primer3 primer design were used to investigate the characteristics of EST-SSR loci and primer development at the graft union of this combination,in order to provide reference for the application of molecular markers and genetic research related to pear grafting.The results showed that the grafting interface transcriptome of the'QAUP-1/Qingzhen D1'grafting combination contained 71 544 unigenes.Taking the longest transcript(number of coding genes)for EST-SSR locus prediction,a total of 2 433 SSR loci were identified,with a frequency of 5.47％in the grafting interface transcriptome of the'QAUP-1/Qingzhen D1'grafting combination(the ratio of the number of detected SSR loci to the total number of unigenes).The most frequently repeated motifs were AG/CT and TC/GA,accounting for 19.40％and 13.93％,respectively.The repeat times of EST-SSR motifs were mainly distributed between 6 and 10,with 1 557 EST-SSR loci in this range,accounting for 63.99％of the total EST-SSR loci.The SSR(simple sequence repeat)loci in the transcriptome at the graft union of the'QAUP-1/Qingzhen D1'grafting combination exhibited good polymorphism.There were 217 SSRs with a length of 10-12 bp,accounting for 8.92％of the total SSR loci.Additionally,there were 1 127 SSRs with lengths ranging from 13-20 bp,representing 46.32％of the total SSR loci.Furthermore,1 089 SSRs with lengths exceeding 20 bp made up 44.76％of the total SSR loci.Twelve pairs of EST-SSR primers were randomly selected,and a total of 59 bands were amplified across five pear tissue samples,with an average of 4.9 allelic loci per EST-SSR and 4 diverse bands.关键词
梨/转录组/EST-SSR/引物开发Key words
pear/transcriptome/EST-SSR/primer development分类
农业科技引用本文复制引用
尹玉环,于心怡,李冰,刘建龙,杨英杰,李鼎立..基于梨转录组的SSR分子标记的开发与应用[J].北方园艺,2026,(2):19-26,8.基金项目
山东省农业良种工程资助项目(2022LZGC011) （2022LZGC011）
国家现代农业产业技术体系建设专项资金资助项目(CARS-29-07) （CARS-29-07）
国家自然科学基金青年基金资助项目(31401832). （31401832）
