北方园艺Issue(2)：27-36,10.DOI:10.11937/bfyy.20252655
狗牙根PIF基因家族鉴定及遮荫胁迫分析
Identification and Shade Stress Response Analysis of the PIF Gene Family in Bermudagrass
摘要
Abstract
Taking bermudagrass(Cynodon dactylon L.)variety'A12359'as the test material,bioinformatics related methods were used to study the PIF gene relationship,gene structure,conserved motifs,promoter cis-acting elements,and gene expression patterns under shading stress,in order to reveal the potential role of this gene family in shading stress response and provide reference for the improvement of bermudagrass varieties and molecular breeding.The results showed that 33 CdPIFs genes were identified in bermudagrass,a relatively high number compared to other species,which may be attributed to its genome duplication history and habitat adaptation.Phylogenetic analysis classified these 33 genes into four subfamilies.Conserved motif analysis demonstrated that most CdPIFs genes contain motif 1,motif 3,and motif 7,indicating high evolutionary conservation.Promoter cis-acting element analysis revealed an enrichment of light-responsive,hormone-responsive,and stress-responsive elements,with light-responsive elements being the most abundant,underscoring the critical role of light in CdPIFs gene regulation.Gene linkage and chromosomal localization analyses indicated an uneven distribution of multiple CdPIF genes across chromosomes.Transcriptomic analysis identified significant differential gene expression in the 6-hour shade treatment group compared to other treatments.Notably,CdPIF15,CdPIF25,CdPIF26,and CdPIF27 exhibited consistently high expression levels under shade stress.关键词
狗牙根/PIF基因/基因家族鉴定/遮荫胁迫Key words
bermudagrass/PIF gene/gene family identification/shade stress分类
农业科技引用本文复制引用
孙晓阳,刘彤生,武轩阳,吴可,付金民..狗牙根PIF基因家族鉴定及遮荫胁迫分析[J].北方园艺,2026,(2):27-36,10.基金项目
国家自然科学基金资助项目(32401481) （32401481）
山东省重点研发资助项目(2024LZGC00303). （2024LZGC00303）
