北方园艺Issue(2):63-70,8.DOI:10.11937/bfyy.20252759
以凋落物为基质栽培肺形侧耳的配方筛选及营养成分分析
Formula Selection and Nutrient Composition of Pleurotus pulmonata Cultivated With Litter as Substrate
摘要
Abstract
The effects of different broad-leaved tree litter on mycelial growth,fruiting body yield and quality of Pleurotus pulmonata were studied by microscopic observation of colony morphology and hyphae,stand-off culture,ITS sequence analysis and plate culture with P.pulmonata strains as test materials,in order to provide reference for expanding the selection range of low-cost cultural substrates and resource utilization of litter of P.pulmonata.The results showed that DL-P.pul001 showed obvious superiority in hyphae growth rate on 7 broad-leaved tree litter substrates,and the growth rate on Tilia amurensis litter was the fastest((4.02±0.19)mm·d-1),significantly faster than that of sawdust control((3.10±0.05)mm·d-1).Furthermore,four formulas were designed to cultivate P.pulmonata DL-P.pul001 with T.amurensis litter as the main substrate.The fruit body yield((133.00±1.55)g·bag-1),stipe diameter((2.24±0.27)cm),capsule length((7.21±0.54)cm),capsule width((6.57±0.36)cm)and crude protein((235.75±0.27)mg·g-1)of P.pulmonata cultivated with formula Ⅲ(55%litter,18%fungus bran,15%wheat bran,10%corncob,1%lime,1%gypsum)were better than those of the sawdust control group.Formula Ⅱ(65%litter,8%fungus chaff,15%wheat bran,10%corncob,1%lime,1%gypsum)was the best in the yield of crude polysaccharide((19.15±0.18)mg·g-1)and crude fat(2.98%±0.09%).关键词
肺形侧耳/凋落物/产量/农艺性状/营养成分Key words
Pleurotus pulmonarius/litter/yield/agronomic traits/nutritional component分类
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孙嫣然,王士元,李亚伟,刘增才,邹莉..以凋落物为基质栽培肺形侧耳的配方筛选及营养成分分析[J].北方园艺,2026,(2):63-70,8.基金项目
黑龙江凉水国家级自然保护区林下凋落物专项调查资助项目(432231010). (432231010)