茶叶学报2025，Vol.66Issue(5)：1-12,12.DOI:10.20045/j.cnki.issn.2096-0220.2025.05.001
灰茶尺蠖耐寒能力及适应机制研究
Cold Tolerance and Adaptation Mechanism of Ectropis Grisescens
摘要
Abstract
[Objectives]Tolerance and adaptability to and outbreak potential in winter of Ectropis grisescens Warren,one of the most widespread and destructive pests at tea plantations in northern China,were studied.[Method]Overwintering ability of E.grisescens at various life stages was determined by measuring the supercooling capacity and low-temperature survival of the moth.Effects of cold hardening the tea geometrids on mortality and contents of moisture,fats,and glycerol as well as the activities of antioxidative catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)were analyzed.[Result]The lowest supercooling point(SCP)and freezing point(FP)of E.grisescens pupae were -18.66℃and -10.56℃,respectively.The cold hardiness of the moth at various developmental stages ranked pupae>4th instar>3rd instar>5th instar larvae.Under acute exposure to -4℃,the median lethal times(LT50)for 3rd to 5th instar larvae were 3.643 h,4.573 h,and 3.671 h,respectively,whereas the pupae could sustain a temperature as low as -7℃with a significantly higher LT50 of 15.513 h.In addition,the survival of the larvae and pupae were significantly enhanced by a 5℃cold hardening treatment.A 6h treatment could result in a 40％reduction in mortality rate from 60％-80％to 20％-40％.The contents of glycerol,a cryoprotectant that increased their tolerance to low temperatures,in the 4th instar,5th instar,and pupae varied significantly by the hardening as well.Meanwhile,the activities of CAT,POD,and SOD rose under the treatment.That the coordinated enhancement mitigated the cytotoxic effect of cold stress on the moth suggested the antioxidant defense system actively participated in the cold hardiness augmentation in E.grisescens.[Conclusion]Even though the tolerance to coldness in winter of E.grisescens larvae was low,the moth could,nonetheless,survive in a plastic-sheltered tea plantation in northern China through cold hardening to emerge in the following spring.关键词
灰茶尺蠖/北方茶园/耐寒能力/冷驯化Key words
Ectropis grisescens/northern tea plantations/cold resistance/cold hardening分类
农业科技引用本文复制引用
陈馨茹,赵桃彦,王璐,李兆群,许永玉,陈珍珍..灰茶尺蠖耐寒能力及适应机制研究[J].茶叶学报,2025,66(5):1-12,12.基金项目
山东省茶叶产业技术体系(SDARS-19-04) （SDARS-19-04）
山东省农业重大技术协同推广计划(SDNYXTTG-2024-30). （SDNYXTTG-2024-30）
