南方医科大学学报2026，Vol.46Issue(2)：247-258,12.DOI:10.12122/j.issn.1673-4254.2026.02.02
党参-茯苓配伍调控ERα/PI3K/Akt信号通路改善单侧颈总动脉永久结扎痴呆大鼠的认知障碍
Radix codonopsis combined with Poria improves cognitive impairment in rats with unilateral common carotid artery ligation by regulating the ERα/PI3K/Akt signaling pathway
摘要
Abstract
Objective To investigate the mechanism of Radix codonopsis combined with Poria(CRP)for improving cognitive impairment induced by permanent unilateral common carotid artery ligation(UCCA)in rats.Methods UPLC-Q-TOF-MS/MS and network pharmacology analysis were used to analyze the components of CRP in the blood and brain of rats medicated with CRP.Rat models of UCCA were treated with CRP gavage for 1 month,and Morris water maze,HE staining,and NeuN staining were used to assess the therapeutic efficacy.Metabolomics analysis,ELISA,immunohistochemistry,and Western blotting were employed to explore and validate the therapeutic mechanism of CRP.Results A total of 125 chemical constituents were identified in the brain tissue(89 Radix codonopsis components and 36 Poria components)and 126 in blood samples(85 Radix codonopsis components and 41 Poria components)of medicated rats.Network pharmacology analysis revealed that the therapeutic mechanism of CRP on dementia involved primarily the PI3K/Akt signaling pathway,regulated by the brain-targeting constituents of CRP.In UCCA rats,CRP treatment significantly improved their performance in Morris water maze test,alleviated neuronal damage in the hippocampus and cortex,and increased expression level of NeuN.Metabolomics analysis revealed significant enrichment of the estrogen and GABAergic synapse signaling pathways in CRP-treated rats.CRP obviously reduced the brain levels of glutamate and GABA/glutamate in UCCA rats,and downregulated the proteins expressions of ERα,p-PI3K/PI3K and p-Akt/Akt.Conclusion CRP improves learning and memory impairment in UCCA rats possibly by modulating the ERα/PI3K/Akt signaling pathway in the brain.关键词
党参-茯苓配伍/痴呆/单侧颈总动脉永久结扎 大鼠/γ-氨基丁酸/ERα/PI3K/Akt信号通路Key words
compatibility of Radix codonopsis and Poria/dementia/permanent unilateral common carotid artery ligation/GABA/ERα/PI3K/Akt signaling pathway引用本文复制引用
杨佳瑶,何玉莲,郭延垒,尚芳红,花雷,阳勇,张小梅,魏江平..党参-茯苓配伍调控ERα/PI3K/Akt信号通路改善单侧颈总动脉永久结扎痴呆大鼠的认知障碍[J].南方医科大学学报,2026,46(2):247-258,12.基金项目
国家自然科学基金青年科学基金(82104427) （82104427）
重庆市巴渝青年岐黄学者支持项目Supported by Natural Science Foundation for the Youth(NSFY)of China(82104427). （NSFY）
