南方医科大学学报2026，Vol.46Issue(2)：271-277,7.DOI:10.12122/j.issn.1673-4254.2026.02.04
植物乳杆菌通过调控胆汁酸代谢并抑制海马体NLRP3蛋白表达缓解铅暴露导致的小鼠学习记忆障碍
Lactobacillus plantarum alleviates lead exposure-induced learning and memory impairment in mice by regulating bile acid metabolism and inhibiting hippocampal NLRP3 expression
摘要
Abstract
Objective To explore the mechanism of Lactobacillus plantarum(L.plantarum)for alleviating learning and memory impairments caused by lead exposure.Methods Twenty-four C57BL/6J mice were randomized equally into control group,lead exposure(100 mg/L)group,and L.plantarum treatment group with daily gavage of L.plantarum(109 CFU)for 10 weeks.Learning and memory abilities of the mice were evaluated using Morris water maze test,and blood lead level and bile acid levels were determined using inductively coupled plasma mass spectrometry(ICP-MS)and liquid chromatography-mass spectrometry(LC-MS),respectively.Hippocampal pathologies of the mice were examined with HE staining,and immunohistochemistry was used to observe morphological changes and microglia activation in the hippocampus.Serum levels of TNF-α and IL-1β of the mice were determined with ELISA,and hippocampal expression levels of NLRP3 and TGR5 proteins were detected using Western blotting.Results Compared with those in lead exposure group,the mice receiving L.plantarum intervention showed significantly increased exploration time,swimming distance,and target platform crossings with reduced latency period to find the hidden platforms in Morris water maze test.L.plantarum intervention partially reversed lead exposure induced reduction of serum levels of deoxycholic acid,3-hydroxydeoxycholic acid,3-hydroxyursodeoxycholic acid,3-thiodeoxycholic acid,and 3-thio-α-methylcholic acid.The treatment also significantly reduced hippocampus pathologies in mice with lead exposure,and reduced their serum levels of IL-1β and TNF-α and hippocampal NLRP3 protein expression levels.Conclusion L.plantarum can alleviate learning and memory impairments caused by lead exposure in mice possibly by regulating bile acid metabolism and inhibiting hippocampal expression of NLRP3 protein.关键词
铅暴露/学习记忆/植物乳杆菌/靶向代谢组学/NLRP3Key words
lead exposure/learning and memory/Lactobacillus plantarum/targeted metabolomics/NLRP3引用本文复制引用
李立凡,余伟健,谭美涛,李韵婷,刘安飞,孟晓静..植物乳杆菌通过调控胆汁酸代谢并抑制海马体NLRP3蛋白表达缓解铅暴露导致的小鼠学习记忆障碍[J].南方医科大学学报,2026,46(2):271-277,7.基金项目
广东省自然科学基金(2023A1515012500,2024A1515012722) （2023A1515012500,2024A1515012722）
