南方医科大学学报2026，Vol.46Issue(2)：286-292,7.DOI:10.12122/j.issn.1673-4254.2026.02.06
黄芪总苷通过抑制P38通路缓解氧糖剥夺/复氧后的星形胶质细胞水肿并降低AQP4表达
Total Astragalus saponins reduce astrocyte swelling and AQP4 expression following oxygen-glucose deprivation and reoxygenation by inhibiting the p38 pathway
摘要
Abstract
Objective To investigate the effect of total Astragalus saponins(AST)on astrocyte swelling induced by glucose-oxygen deprivation and reoxygenation(OGD/R)and its molecular mechanism.Methods Primary astrocytes isolated from the brain of post-natal day 2 SD rats were treated with solvent vehicle,AST,or AST combined with U-46619(a p38 activator)24 h prior to and during exposure to OGD/R.The changes in cell volume and morphology were observed,and intracellular reactive oxygen species(ROS)levels and lactate dehydrogenase(LDH)activity were detected.The expression of aquaporin 4(AQP4)on cell membrane was observed using immunofluorescence confocal microscopy,and Western blotting was performed to detect the changes in p38 signaling pathway phosphorylation levels and AQP4 expression level.Results AST treatment significantly reduced OGD/R-induced increase in astrocyte volume,ROS levels and LDH release.The AST-treated cells also exhibited improved cell morphology,reduced aggregation of AQP4 on the cell membrane,and decreased p38 phosphorylation and AQP4 expression levels.The protective effects of AST against OGD/R-induced cell injuries were significantly attenuated by combined treatment of the cells with U-46619.Conclusion AST alleviate astrocyte swelling induced by OGD/R injury by inhibiting the activation of the p38 signaling pathway and AQP4 overexpression on the cell membrane.关键词
黄芪总苷/星形胶质细胞肿胀/水通道蛋白4/P38/氧糖剥夺/复氧Key words
total Astragalus glycosides/astrocyte swelling/aquaporin 4/p38 signaling pathway/oxygen-glucose deprivation/reoxygenation引用本文复制引用
尹钦,雷雨逍,王晓澍,杨刚,唐兆华..黄芪总苷通过抑制P38通路缓解氧糖剥夺/复氧后的星形胶质细胞水肿并降低AQP4表达[J].南方医科大学学报,2026,46(2):286-292,7.基金项目
国家自然科学基金(81301630) （81301630）
重庆市自然科学基金一般项目(cstc2021jcyj-msxmX0262) （cstc2021jcyj-msxmX0262）
重庆医科大学未来医学青年创新计划(W0106)Supported by National Natural Science Foundation of China(81301630). （W0106）
