南方医科大学学报2026，Vol.46Issue(2)：293-300,8.DOI:10.12122/j.issn.1673-4254.2026.02.07
化瘀通便汤通过抑制PI3K/Akt信号通路促进慢传输型便秘大鼠肠道Cajal间质细胞的胞葬作用
Huayu Tongbian Decoction promotes efferocytosis of interstitial Cajal cells in rats with slow transit constipation by inhibiting the PI3K/Akt signaling pathway
摘要
Abstract
Objective To explore the mechanism of Huayu Tongbian Decoction(HYTBD)for relieving slow transit constipation(STC)in rats.Methods Sixty SD rat models of STC induced by intragastric administration of loperamide hydrochloride suspension were randomized equally into 5 groups for treatment with daily gavage with saline(STC model group),low-,medium-,or high-dose HYTBD,or mosapride(positive control group),with another 12 normal rats receiving saline gavage as the blank control group.The first black stool time,fecal water content and intestinal propulsion rate of the rats were measured,and the ultrastructure of interstitial Cajal cells(ICCs)was observed using transmission electron microscopy to calculate the mitochondrial vacuolar rate.The phagocytosis rate of macrophages was detected using immunofluorescence assay,and colonic pathologies were examined using HE staining.The colonic expression levels of c-Kit,CD47,PI3K and Akt were detected with immunohistochemistry or Western Blotting,and their correlations with efferocytosis were analyzed.Results The rat models of STC showed significant ultrastructural damage of the ICCs with increased first black stool time,mitochondrial vacuolar rate,and colonic expression levels of CD47,p-PI3K/PI3K and p-Akt/Akt proteins,lowered fecal water content,intestinal propulsion rate and c-Kit expression level,without significant changes in phagocytic rate.Treatment with HYTBD,especially at the high dose,significantly increased fecal water content,intestinal propulsion rate,phagocytosis rate and colonic c-Kit expression,and decreased the first black stool time,mitochondrial vacuolar rate,and colonic expressions of CD47,p-PI3K/PI3K and p-Akt/Akt in the mouse models.Conclusion HYTBD improves STC in rats possibly by down-regulating colonic CD47 expression and improving macrophage phagocytosis rate via inhibiting PI3K and Akt phosphorylation,thereby promoting efferocytosis of the ICCs.关键词
化瘀通便汤/胞葬作用/慢传输型便秘/PI3K/Akt信号通路/Cajal间质细胞Key words
Huayu Tongbian Decoction/efferocytosis/slow transit constipation/PI3K/Akt signaling pathway/interstitial Cajal cells引用本文复制引用
邱佳惠,陈萌,满如,陈鑫,邱东瑞,常启同,马洪玉..化瘀通便汤通过抑制PI3K/Akt信号通路促进慢传输型便秘大鼠肠道Cajal间质细胞的胞葬作用[J].南方医科大学学报,2026,46(2):293-300,8.基金项目
国家自然科学基金青年科学基金(81804094) （81804094）
辽宁省教育厅(JYTMS20231814) （JYTMS20231814）
辽宁省教育厅高校基本科研项目储备项目(2024-JYTCB-001)Supported by Natural Science Foundation for the Youth(NSFY)of China(81804094). （2024-JYTCB-001）
