南方医科大学学报2026，Vol.46Issue(2)：316-324,9.DOI:10.12122/j.issn.1673-4254.2026.02.09
异牡荆素通过促进SIRT3表达减轻糖尿病小鼠的心肌氧化应激损伤
Isovitexin alleviates myocardial oxidative stress injury in diabetic mice by enhancing myocardial SIRT3 expression and reducing oxidative stress
摘要
Abstract
Objective To investigate the protective effect of isovitexin(ISO)on the myocardium of diabetic mice and explore its mechanism.Methods Eighteen adult male C57 mice were randomly divided into control group,diabetes mellitus(DM)group and DM+ISO group(n=6).Primary cultures of neonatal mouse cardiomyocytes(NMCMs)were exposed to high glucose(33 mmol/L)and treated with ISO alone or in combination with 3-TYP(a SIRT3 inhibitor).HE staining was used to evaluate myocardial injury in the diabetic mice,and the levels of MDA,SOD and GSH-Px in the myocardium were detected with ELISA.The expressions of iNOS,NOX2 and 8-OHdG were detected using immunohistochemistry and fluorescence staining.Western blotting was used to analyze the expression levels of NRF2,NOX2,NQO1,and SIRT3,and ROS levels were determined with flow cytometry.Results The diabetic mice showed obvious inflammatory cell infiltration in the myocardium,increased myocardial levels of IL-1β,IL-6 and TNF-α,decreased expression levels of NQO1,NRF2 and SIRT3 proteins,and increased expression levels of NOX2 and AC-SOD2 proteins.ISO treatment of the diabetic mice significantly reduced myocardial inflammatory cell infiltration,lowered the levels of IL-1β,IL-6 and TNF-α,restored the protein levels of NQO1,NRF2 and SIRT3,and decreased the protein levels of NOX2 and AC-SOD2.Conclusion ISO can alleviate diabetic myocardial injury in mice by promoting SIRT3 expression and reducing oxidative stress.关键词
异牡荆素/糖尿病/心肌病/氧化应激/SIRT3Key words
isovitexin/diabetes mellitus/cardiomyopathy/oxidative stress/SIRT3引用本文复制引用
彭煜策,姜毅,马丹,何岸,吕鼎一,罗明昊,罗素新..异牡荆素通过促进SIRT3表达减轻糖尿病小鼠的心肌氧化应激损伤[J].南方医科大学学报,2026,46(2):316-324,9.基金项目
国家自然科学基金(82400477) （82400477）
重庆市自然科学基金面上项目(CSTB2024NSCQ-MSX1212) （CSTB2024NSCQ-MSX1212）
重庆市医学人才-青年拔尖人才项目(YXQN2025067)Supported by National Natural Science Foundation of China(82400477). （YXQN2025067）
