广东医学2026，Vol.47Issue(1)：23-28,6.DOI:10.13820/j.cnki.gdyx.20252593
MinION测序技术快速检测结核分枝杆菌复合群的敏感度和特异度评估
Rapid detection of mycobacterium tuberculosis complex in sputum specimens using MinION sequencing:sensitivi-ty and specificity evaluation
摘要
Abstract
Objective To evaluate the sensitivity,specificity,and diagnostic performance of MinION sequencing technology for rapid detection of Mycobacterium tuberculosis complex(MTBC)in sputum specimens,compared with acid-fast staining microscopy and quantitative PCR(qPCR),and to assess its stability and reproducibility for potential clinical application.Methods Sputum samples were spiked with varying concentrations of M.tuberculosis H37Rv stand-ard strain to simulate clinical infection and analyzed using acid-fast staining,qPCR,and MinION sequencing to deter-mine sensitivity.To assess specificity,sputum samples were spiked with different non-tuberculous mycobacteria species,DNA was extracted,and analyzed via MinION sequencing.The minimum detection limit,reproducibility,and cross-re-activity were evaluated.Results MinION sequencing detected MTBC at a minimum concentration of 104 cfu/mL with a specificity of 100％.However,reproducibility was suboptimal,and detection thresholds were relatively high compared to conventional methods.Conclusion MinION sequencing enables direct detection of MTBC in sputum samples with high specificity but exhibits limited reproducibility and sensitivity at lower bacterial loads.Further validation and data are re-quired before routine clinical implementation.关键词
MinION测序/结核分枝杆菌复合群检测/敏感度/特异度Key words
MinION sequencing/detection of Mycobacterium tuberculosis complex/sensitivity/specificity分类
医药卫生引用本文复制引用
夏良华,于圣铭,谢伶,晏亮,陈开森,谢荣慧..MinION测序技术快速检测结核分枝杆菌复合群的敏感度和特异度评估[J].广东医学,2026,47(1):23-28,6.基金项目
国家自然科学基金(82060611) （82060611）
江西省科技合作一般项目(20212BDH81017) （20212BDH81017）
