干旱地区农业研究2026，Vol.44Issue(1)：31-40,10.DOI:10.7606/j.issn.1000-7601.2026.01.04
藜麦DOF基因家族鉴定及NO调控干旱胁迫下表达模式分析
Identification of the DOF gene family in quinoa(Chenopodium quinoa)and analysis of expression patterns regulated by nitric oxide(NO)under drought stress
摘要
Abstract
DOF(DNA binding with one zinc finger)is a plant-specific transcription factor that plays a crucial role in plants' response to abiotic stress.Through bioinformatics methods,the DOF gene family members in quinoa were identified genome-wide,and the expression patterns of this gene family under drought stress regulated by exog-enous NO were analyzed using qRT-PCR technology.The results showed that a total of 25 family members of the quinoa DOF gene family were identified,with amino acid lengths ranging from 143 to 558 aa,molecular weights ranging from 16 079.9 to 59 102.2 Da,isoelectric points ranging from 4.52 to 9.60,hydrophobic indices less than 2,and all predicted to be located in the nucleus.Phylogenetic analysis divided the DOF gene family into five sub-families.The 25 DOF genes were evenly distributed on 13 chromosomes,with 6 pairs of tandem repeat genes.There were 31 cfs-acting elements related to light response,tissue specificity,stress response,and plant hormones in the promoter region.DOF5.3 was the core node of the protein interaction network.Expression analysis in different tis-sues and organs indicated that CqDOFs were expressed in all quinoa tissues,with stronger expression in flowers and leaves.Under different abiotic stress conditions,most CqDOFs genes responded strongly in roots.qRT-PCR analysis showed that under drought stress regulated by exogenous NO,the relative expression levels of 16 CqDOFs genes significantly increased.Among them,the expression level of CqDOF10 under drought stress regulated by NO was 215.3 times that under drought stress alone,indicating that CqDOFs genes play an important role in the regula-tory mechanism of quinoa's response to drought stress,and exogenous NO can effectively increase the expression of genes under drought stress.关键词
藜麦/DOF基因家族/NO/干旱胁迫/生物信息学/表达模式Key words
quinoa/DOF gene family/NO(nitric oxide)/drought stress/bioinformatics/expression pattern分类
农业科技引用本文复制引用
王雪玮,王宝强,朱晓林,桂金凤,陈立飞,魏小红..藜麦DOF基因家族鉴定及NO调控干旱胁迫下表达模式分析[J].干旱地区农业研究,2026,44(1):31-40,10.基金项目
国家自然科学基金(32060401) （32060401）
甘肃省自然科学基金(23JRRA1426) （23JRRA1426）
