干旱地区农业研究2026，Vol.44Issue(1)：62-70,9.DOI:10.7606/j.issn.1000-7601.2026.01.07
叶面喷施'维大力'缓解蚕豆幼苗肉桂酸胁迫的生长生理效应
Growth and physiological effects of foliar application of VDAL on alleviating cinnamic acid stress in fava bean seedlings
摘要
Abstract
To explore the alleviation effects and appropriate application concentration of foliar application of Verticillium dahliae Asp-f2 like protein(VDAL)on the damage to fava bean seedlings under autotoxicity stress in-duced by cinnamic acid(CA),the potted seedlings of'Qingcan 13'were used as the experimental material,and different concentrations of VDAL(1.0,1.5,2.0,2.5 mg·L-1,and 3.0 mg·L-1)were set for foliar application by using 0.9 mmol·L-1 CA to simulate the autotoxicity in continuous cropping obstacles.The growth and physiolog-ical responses of the fava bean seedlings were investigated.The results indicated that CA stress significantly inhibi-ted the growth of faba bean seedlings,and foliar spraying of 2.5 mg·L-1 DAL could effectively alleviate its toxic effects.Compared with the CA treatment,2.5 mg·L-1 VDAL significantly increased the plant height,leaf area,shoot dry biaomass and root dry biaomass of faba bean seedlings by 104.88％,58.39％,115.71％and 103.85％,respectively.In terms of photosynthetic characteristics,the contents of leaf total chlorophyll and carotenoids,as well as net photosynthetic rate,transpiration rate and stomatal conductance under 2.5 mg·L-1 VDAL treatment were increased by 73.88％,136.82％,59.03％,596.25％and 132.68％,respectively.At the physiological index level,2.5 mg·L-1 VDAL significantly decreased the intercellular CO2 concentration,malondialdehyde content,and hydrogen peroxide content by 31.04％,40.68％and 35.43％,respectively,while it increased the contents of proline,soluble sugar and soluble protein,as well as the activities of superoxide dismutase,peroxidase,catalase and ascorbate peroxidase by 73.18％,27.08％,33.54％,16.99％,145.58％,102.82％and 96.20％,respectively.In conclusion,foliar spraying with appropriate concentration of VDAL could improve the photosynthetic rate,osmot-ic regulation ability and antioxidant capacity of faba bean seedlings under cinnamic acid stress,effectively alleviate the continuous cropping obstacle,with the optimal effect observed at a concentration of 2.5 mg·L-1.关键词
蚕豆/维大力(大丽花轮枝孢Asp-f2类蛋白)/肉桂酸/自毒作用/连作障碍Key words
fava bean/VDAL(Verticillium dahliae Asp-f2 like protein)/cinnamic acid(CA)/autotoxicity/continuous cropping obstacle分类
农业科技引用本文复制引用
刘秀娟,杨文欢,曹金萍,王玉萍..叶面喷施'维大力'缓解蚕豆幼苗肉桂酸胁迫的生长生理效应[J].干旱地区农业研究,2026,44(1):62-70,9.基金项目
甘肃省重点研发项目(25YFNA035) （25YFNA035）
甘肃省科技特派员(团)专项(25CXNN001) （团）
国家现代农业产业技术体系(CARS-08) （CARS-08）
