南方农业学报2025，Vol.56Issue(11)：3307-3319,13.DOI:10.3969/j.issn.2095-1191.2025.11.001
航天搭载下竹叶花椒遗传变异与果实代谢变化分析
Analysis of genetic variations and fruit metabolic changes in Zanthoxylum armatum under spaceflight conditions
摘要
Abstract
[Objective]This study aimed to analyze genetic variations and fruit metabolic changes in Zanthoxylum armatum under spaceflight conditions,to provide references for screening and identifying space-mutagenized Zanthoxylum armatum populations,germplasm innovation and new cultivar breeding of prickly ash.[Method]Two Zanthoxylum arma-tum cultivars,Zaoshujiuyeqing(ZSJ)and Tengjiao(TJ)were used as research subjects,and the non-mutagenized samples as control(CK).Based on reference genomes of Zanthoxylum armatum,genome resequencing,and untargeted metabolomic analysis were utilized to perform genomic mutation detection and analysis of pre-spaceflight samples before(ZSJ-CK and TJ-CK)and post-spaceflight samples(ZSJ-HT and TJ-HT).The GO functional annotation and KEGG sig-naling pathway analysis were performed on mutant genes,and KEGG metabolic pathway enrichment analysis were per-formed on differential metabolites in pre-spaceflight and post-spaceflight samples.[Result]The single nucleotide variant(SNV)number of ZSJ-HT samples was 1.76 times that of ZSJ-CK samples;the insertion/deletion variants(InDel)num-ber of ZSJ-HT samples was 2.12 times that of ZSJ-CK samples.For mutation number of InDel at each mutant fragment length,the InDel number of ZSJ-HT samples was higher than that of ZSJ-CK samples,while the InDel number of TJ-HT samples was higher than that of TJ-CK samples.In the groups of ZSJ-HT vs ZSJ-CK and TJ-HT vs TJ-CK,the total num-bers of SNVs were 65150 and 68473,the total numbers of InDels were 1615 and 2430,and the numbers of protein-coding genes with meaningful mutations were 936 and 1034,respectively.GO functional terms with the highest number of annotated mutated genes in the groups of ZSJ-HT vs ZSJ-CK and TJ-HT vs TJ-CK included metabolic processes,or-ganic substance metabolic processes,integral component of membrane,and zinc ion binding.The KEGG signaling path-ways with the largest number of mutant genes were metabolic pathways,biosynthesis of secondary metabolites,plant-pathogen interaction,and plant hormone signal transduction.In the ZSJ-HT vs ZSJ-CK group,the contents of 264 me-tabolites significantly increased,while the contents of 325 metabolites significantly decreased.In the TJ-HT vs TJ-CK group,the contents of 398 metabolites significantly increased,and the contents of 271 metabolites significantly de-creased.KEGG metabolic pathway enrichment analysis revealed that the most enriched pathways in the ZSJ-HT vs ZSJ-CK group were flavonoid and flavonol biosynthesis and flavonoid biosynthesis,whereas the most enriched pathways in the TJ-HT vs TJ-CK group were ABC transporters and phenylpropanoid biosynthesis.[Conclusion]Spaceflight conditions induce considerable changes in all protein-coding genes in genomes and metabolite contents and compositions of mature fruits,and the screened metabolic pathways with significant enrichment and significant differential metabolites can be used in further screening and identification of mutagenized Zanthoxylum armatum populations.关键词
竹叶花椒/航天搭载/诱变育种/基因组/代谢组Key words
Zanthoxylum armatum/spaceflight/mutagenesis breeding/genome/metabolome分类
农业科技引用本文复制引用
黄明,张义刚,吴纯清,罗友进,程玥晴,胡佳羽,谢永红,陈霞..航天搭载下竹叶花椒遗传变异与果实代谢变化分析[J].南方农业学报,2025,56(11):3307-3319,13.基金项目
国家重点研发计划项目(2021YFD1000405) （2021YFD1000405）
重庆市现代农业产业技术体系创新团队项目(CQMAITS202410) （CQMAITS202410）
重庆市留学人员回国创业创新支持计划项目(2024) （2024）
重庆市农业科学院市级财政专项科技创新类项目(2024) National Key Research and Development Program of China(2021YFD1000405) （2024）
Innovation Team Building Project of Chongqing Modern Agricultural Industrial Technology System(CQMAITS202410) （CQMAITS202410）
Chongqing Municipal Returning Overseas Scholars Entrepreneurship and Innovation Support Program(2024) （2024）
Municipal Financial Special Fund Project for Scientific and Technological Innovation of Chongqing Academy of Agricultural Sciences(2024) （2024）
