南方农业学报2025，Vol.56Issue(11)：3320-3331,12.DOI:10.3969/j.issn.2095-1191.2025.11.002
盐胁迫下不同亚种水稻种质资源的根系形态特征分析
Analysis of root morphology characteristics of germplasm resources of different rice subspecies under salt stress
摘要
Abstract
[Objective]This study aimed to analyze the root morphological characteristics of different rice subspecies under salt stress,elucidate their coping strategies,and identify germplasm resources with excellent root characteristics un-der salt stress,providing a theoretical basis for breeding salt-tolerant and high-yield rice varieties.[Method]Using 23 in-dica,35 japonica,and 17 AUS rice germplasm resources as experimental materials,and a hydroponic experiment of salt stress treatment at the seedling stage(adding 125 mmol/L NaCl to rice nutrient solution)was conducted,and 12 root mor-phological indexes were measured.Multiple statistical methods,including correlation analysis,principal component analy-sis(PCA),linear regression,and membership function were employed to comprehensively evaluate root morphological characteristics of different rice subspecies under salt stress.[Result]Among different rice subspecies,indica rice exhibited the highest mean values of salt tolerance level(5.0),followed by japonica rice(4.0)and AUS rice(3.6).The root total number,total length of 0-0.5 mm root,total length of 0.5-1.0 mm root,total root surface area,and total root volume of indica rice were extremely significantly higher than those of japonica rice and AUS rice(P<0.0001).Correlations among the 12 root traits varied across the three rice subspecies.In PCA,two principal components for each subspecies were extracted,with cumulative contribution rates of 85.638%for indica rice,83.277%for japonica rice,and 80.342%for AUS rice.Linear regression identified four key indexes of root morphology under salt stress for different rice subspecies:for indica rice were root total number,total length of 0.5-1.0 mm root,total root surface area,and total root volume;for ja-ponica rice were total root surface area,total length of 0.5-1.0 mm root,total surface area of 0-0.5 mm root,and root total number;for AUS were total volume of 0-0.5 mm root,total volume of 0.5-1.0 mm root,total root volume,and root average diameter.Through comprehensive evaluation of D values and salt tolerance level,salt-tolerant germplasms with excellent root morphological characteristics were identified in each subspecies:Vary Vato 462(indica rice),Ta Mao Tsao(japonica rice),and DJ 24(AUS rice).[Conclusion]Under salt stress,indica rice generally outperform japonica rice and AUS rice in root morphological traits,with obviously different coping strategies among them.关键词
盐胁迫/水稻亚种/种质资源/根系形态特征/应对策略Key words
salt stress/rice subspecies/germplasm resources/root morphological characteristics/coping strategies分类
农业科技引用本文复制引用
刘松瑞,陈敏,许昊,王敬文,董悦,马东花,罗成科,李培富,田蕾..盐胁迫下不同亚种水稻种质资源的根系形态特征分析[J].南方农业学报,2025,56(11):3320-3331,12.基金项目
国家自然科学基金项目(32260492) （32260492）
国家重点研发计划项目(2021YFD1900603) （2021YFD1900603）
宁夏粮食作物种质创制与生长调控科技创新团队项目(2022BSB03109) National Natural Science Foundation of China(32260492) （2022BSB03109）
National Key Research and Develop-ment Program of China(2021YFD1900603) （2021YFD1900603）
Ningxia Grain Crop Germplasm Creation and Growth Regulation Science and Technology Innovation Team Project(2022BSB03109) （2022BSB03109）
