海南医科大学学报2026，Vol.32Issue(2)：89-95,7.DOI:10.13210/j.cnki.jhmu.20250225.001
基于周期阻滞和氧化-抗氧化失衡研究槟榔碱致小鼠骨髓细胞DNA损伤的机制
Mechanism of DNA damage in mouse bone marrow cells induced by arecoline based on cycle arrest and oxidation antioxidant imbalance
摘要
Abstract
Objective:To study the DNA damage effect of arecoline(ARC)on mouse bone marrow cells in vivo,and to ex-plore its mechanism.Methods:Single cell gel electrophoresis was used to analyze the DNA damage effect of ARC on mouse bone marrow cells.Flow cytometry,DCFH-DA fluorescence staining,and Rhodamine 123 staining were used to analyze the cell cycle,superoxide dismutase(SOD)activity,glutathione(GSH),malondial dehyde(MDA),and reactive oxygen species(ROS)content,mitochondrial transmembrane potential(Δψm)and p53 protein content of mouse bone marrow cells.Results:ARC,at a dose of 1/2 LD50,could induce DNA damage in mouse bone marrow cells.The difference was significant compared to the negative control group.Cell cycle changed observably in a dose-dependent manner when exposed to ARC.The percentage of cells in G0/G1 phase was significantly increased(P<0.01),and ARC caused G0/G1 phases' cell cycle arrest in mouse bone marrow cells.After the ad-ministration of ARC,SOD activity and GSH content were significantly decreased(P<0.01),MDA and ROS contents were sig-nificantly increased(P<0.01).Furthermore,the Δψm was significantly reduced with increasing dose(P<0.01).The protein lev-els of p53 in mouse bone marrow cells was increased in a dose-dependent manner after ARC treatment(P<0.01).Conclusion:ARC can cause DNA damage of mouse bone marrow cells,increase the expression of p53 protein causing cell cycle arrest in the G0/G1 phase,decrease Δψm,increase ROS in mouse bone marrow cells,enhance lipid peroxidation,severely damage cellular an-tioxidant function,and lead to the imbalance of oxidation and antioxidation.These are the mechanisms of DNA damage in mouse bone marrow cells caused by ARC.关键词
槟榔碱/骨髓细胞/DNA损伤/G0/G1期阻滞/氧化-抗氧化失衡Key words
Arecoline/Bone marrow cell/DNA damage/G0/G1 phase arrest/Imbalance of oxidation-antioxidation分类
医药卫生引用本文复制引用
于蕾,于晶涵,宋辉,郎朗,马瑗晗,龚艳朝,张小坡,张彩云,王正文..基于周期阻滞和氧化-抗氧化失衡研究槟榔碱致小鼠骨髓细胞DNA损伤的机制[J].海南医科大学学报,2026,32(2):89-95,7.基金项目
This study was supported by the National Natural Science Foundation of China project(82060778) （82060778）
Hainan Provincial Natural Science Foundation of China(820RC776) （820RC776）
Hainan Province Health Technology Innovation Joint Project(WSJK2024MS162) （WSJK2024MS162）
Heilongjiang Province Traditional Chinese Medicine Research Project(ZHY2024-098). 国家自然科学基金(82060778) （ZHY2024-098）
海南省自然科学基金(820RC776) （820RC776）
海南省卫生健康科技创新联合项目(WSJK2024MS162) （WSJK2024MS162）
黑龙江省中医药科研课题项目(ZHY2024-098) （ZHY2024-098）
