海南医科大学学报2026，Vol.32Issue(2)：96-102,7.DOI:10.13210/j.cnki.jhmu.20250326.001
ACC通过调控GluN2B/CaMKⅡ信号通路改善精神分裂症模型小鼠的认知功能障碍
ACC improves cognitive dysfunction in schizophrenia model mice by regulating the GluN2B/CaMKⅡ signaling pathway
摘要
Abstract
Objective:To investigate the effects of 1-aminocyclopropanecarboxylic acid(ACC)on behavioral abnormalities and the underlying molecular mechanisms in a schizophrenia(SCZ)model induced by dizocilpine maleate(MK801)in mice.Meth-ods:Eight-week-old male C57BL/6J mice were randomly divided into 3 groups:the normal control group,the SCZ model group,and the ACC intervention group.The schizophrenia model was established via intraperitoneal injection of MK801.Behav-ioral changes were evaluated using the open field test and the novel object recognition test.Western blot was utilized to assess the expression levels of GluN2B,CaMKⅡ,and p-CaMKⅡ(Thr305)proteins in the hippocampus.Additionally,molecular docking studies were conducted to explore the binding characteristics between ACC and GluN2B subunit of the N-methyl-D-aspartate re-ceptor(NMDAR).Results:Behavioral assessments revealed that ACC specifically alleviated MK801-induced hyperlocomotion and significantly improved novel object recognition memory deficits.Molecular analyses demonstrated that ACC treatments upreg-ulated the MK801-induced downregulation of GluN2B expression and restored CaMKⅡ phosphorylation levels.Furthermore,mo-lecular docking results indicated stable binding between ACC and GluN2B-NMDAR.Conclusion:ACC may enhance cognitive function in schizophrenia model mice by modulating the GluN2B/CaMKⅡ signaling pathway.关键词
精神分裂症/行为学实验/地卓西平马来酸盐/N-甲基-D-天冬氨酸受体/认知功能障碍Key words
Schizophrenia/Behavioral tests/Dizocilpine maleate/N-methyl-D-aspartate receptor/Cognitive dysfunction分类
医药卫生引用本文复制引用
李振莹,冯仁军,王鹏,王杰,田梦思,刘坤泽,郭青云,温石磊,倪盼丽,卢利方..ACC通过调控GluN2B/CaMKⅡ信号通路改善精神分裂症模型小鼠的认知功能障碍[J].海南医科大学学报,2026,32(2):96-102,7.基金项目
This study was supported by the Key Research and Development Project of Hainan Province(ZDYF2022SHFZ310) （ZDYF2022SHFZ310）
Hainan Provincial Natural Science Foundation of China(821MS0775,8823RC493,821QN261) 海南省重点研发项目(ZDYF2022SHFZ310),海南省自然科学基金项目(821MS0775,823RC493,821QN261) （821MS0775,8823RC493,821QN261）
