海南医科大学学报2026，Vol.32Issue(2)：103-111,9.DOI:10.13210/j.cnki.jhmu.20250208.007
依达拉奉右莰醇对CRS小鼠焦虑抑郁行为的影响和机制研究
Research on the impact and mechanism of edaravone dexborneol on anxiety and depression behaviors in CRS mice
摘要
Abstract
Objective:To investigate the effects of edaravone borneol(EDB)on anxiety and depressive behaviors in chronic restraint stress(CRS)mice and its potential mechanisms.Methods:Male C57BL/6 mice were randomly assigned to 4 groups(n=8):Control group,Chronic restraint stress(CRS)group,EDB[CRS+EDB(5 mg/kg)]group,and EDB[CRS+EDB(10 mg/kg)]group.The depression model was induced by restraining the mice for 4 hours daily for 21 consecutive days.Anxiety and depressive behaviors were assessed using the open field test,tail suspension test,and forced swimming test.qPCR was used to de-tect the expression of GSDMD,IL-1β,AKT,p62,Beclin1,and LC3Ⅱ mRNA.Western blot was employed to measure the pro-tein expression of p-AKT,p-AMPK,p62,Beclin1,LC3B,GSDMD,GSDMD-N,Pro-IL-1β,IL-1β,and BDNF.Immunoflu-orescence was used to observe and count the expression of the neuronal-specific nuclear protein(NeuN)in the hippocampus.Re-sults:In the open field test,the total distance travelled,time spent in the center zone,and average speed of movement in CRS mice were significantly lower than those in the Control group(P<0.05),while these parameters were higher in EDB-treated groups compared to the CRS group(P<0.05).In the tail suspension and forced swimming tests,the immobility time in CRS mice was significantly longer than that in the Control group(P<0.05),while it was shorter in EDB-treated groups compared to the CRS group(P<0.05).qPCR results showed that compared to the Control group,the expression of GSDMD,IL-1β,and p62 mRNA increased,while the expression of Beclin1 and LC3Ⅱ mRNA decreased in the CRS group(P<0.05).Compared to the CRS group,the expression of GSDMD,IL-1β,and p62 mRNA decreased,while the expression of Beclin1 and LC3Ⅱ mRNA in-creased in EDB-treated groups(P<0.05).Western blot results indicated that compared to the Control group,the expression of p-AKT,p62,GSDMD,GSDMD-N,Pro-IL-1β,and IL-1β increased,while the expression of p-AMPK,Beclin1,LC3B,and BDNF decreased in the CRS group(P<0.05).Compared to the CRS group,the expression of p-AKT,p62,GSDMD,GSDMD-N,Pro-IL-1β,and IL-1β decreased,while the expression of p-AMPK,Beclin1,LC3B,and BDNF increased in EDB-treated groups(P<0.05).Immunofluorescence results showed that the number of NeuN in the CA1,CA3,and DG regions of the hippocampus was significantly lower in the CRS group than in the Control group(P<0.05),while it was significantly high-er in EDB-treated groups compared to the CRS group(P<0.05).Conclusion:EDB may improve anxiety and depressive behav-iors in CRS-induced mice by regulating autophagy through the AKT/AMPK pathway,inhibiting pyroptosis and inflammatory re-sponses,and reducing neuronal damage.关键词
依达拉奉右莰醇/焦虑抑郁/慢性约束应激/自噬/焦亡/神经元特异性核蛋白(NeuN)Key words
Edaravone dexborneol/Anxiety and Depression/Chronic Restraint Stress/Autophagy/Pyroptosis/NeuN分类
医药卫生引用本文复制引用
李文沛,胡琼琼,徐志强,李毅娣,付娟,杨改清..依达拉奉右莰醇对CRS小鼠焦虑抑郁行为的影响和机制研究[J].海南医科大学学报,2026,32(2):103-111,9.基金项目
This study was supported by Henan Province Natural Science Foundation for Young Scientists(242300421515) （242300421515）
Key Scientific Research Project of Henan Province Colleges and Universities(24A320029) （24A320029）
Henan Province Medical Science and Technology Public Welfare Project-Provincial and Ministerial Co-construction Project(SB201903033) （SB201903033）
Henan Province Science and Technology Tackling Project(192102310082) 河南省自然科学基金青年科学基金项目(242300421515) （192102310082）
河南省高等学校重点科研项目(24A320029) （24A320029）
河南省医学科技公关计划省部共建项目(SB201903033) （SB201903033）
河南省科技攻关项目(192102310082) （192102310082）
