海南医科大学学报2026，Vol.32Issue(2)：128-136,9.DOI:10.13210/j.cnki.jhmu.20250102.005
白头翁皂苷B4通过调控AMPK/ACC/CPT1A信号通路改善非酒精性脂肪肝小鼠氧化应激水平的研究
Study of anemoside B4 improving oxidative stress levels in mice with non-alcoholic fatty liver disease by regulating the AMPK/ACC/CPT1A signaling pathway
摘要
Abstract
Objective:To explore the intervention effect of anemoside B4(AB4)on mice with non-alcoholic fatty liver disease(NAFLD)and its possible molecular mechanism.Methods:The NAFLD mouse model was established by a high-fat diet and was treated with AB4.During the experiment,the body weight,glucose tolerance,and insulin tolerance of the mice were monitored.After 8 weeks,serum,liver and other tissue samples were collected to detect total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C);Histopathological changes and lipid deposits in the liver were assessed using H&E staining and oil red O staining.Immunofluorescence was used to evaluate the effects of AB4 on liver metabolic inflammation.The mRNA and protein expression levels of adenosine 5'-monophosphate-activated protein kinase(AMPK),acetyl CoA carboxylase(ACC),and carnitine palmitoyltransferase 1A(CPT1A)in liver were detected by qRT-PCR and Western blot.The levels of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)were analyzed.Results:AB4 could significantly reduce the body weight of high-fat fed mice,improve the abnormal glucose tolerance of mice,and relieve insulin resistance.AB4 could improve the pathological changes of TC,TG,LDL-C,and HDL-C.The liver pathological sections of each administration group showed that the degree of injury was reduced,the number of hepatic fat vacuoles was reduced,the serum MDA level was decreased,the SOD and GSH-Px levels were increased,and the expression of liver inflammatory factor interleukin-6 was decreased.In addition,after AB4 interven-tion,the mRNA and protein expressions of AMPK,ACC,and CPT1A in liver of drug administration groups were increased.Conclusion:AB4 can regulate liver fat metabolism and blood glucose level,reduce oxidative stress response,and its potential mechanism of action in the treatment of NAFLD may be related to the regulation of the AMPK/ACC/CPT1A signaling pathway.关键词
白头翁皂苷B4/非酒精性脂肪肝/氧化应激/AMPK/ACC/CPT1A信号通路Key words
Anemoside B4/Non-alcoholic fatty liver disease(NAFLD)/Oxidative stress response/AMPK/ACC/CPT1A signaling pathway分类
医药卫生引用本文复制引用
蒋鸣,田冲冲..白头翁皂苷B4通过调控AMPK/ACC/CPT1A信号通路改善非酒精性脂肪肝小鼠氧化应激水平的研究[J].海南医科大学学报,2026,32(2):128-136,9.基金项目
This study was supported by the 2023 Key Project of Medical Research of Jangsu Yancheng Commission of Health(YK2023032) 江苏省盐城市卫生健康委员会2023年度医学科研立项项目(YK2023032) （YK2023032）
