海南医科大学学报2026，Vol.32Issue(2)：137-147,11.DOI:10.13210/j.cnki.jhmu.20250107.003
靶向HMGCS1探讨对宫颈癌细胞线粒体基因表达调控的作用机制
Targeting HMGCS1 to explore the mechanism of action on the regulation of mitochondrial gene expression in cervical cancer cells
摘要
Abstract
Objective:To investigate the mechanism of the regulatory effect of 3-hydroxy-3-methylglutaryl coenzyme A syn-thase 1(HMGCS1)on mitochondrial gene expression in cervical cancer(CC)cells.Methods:Bioinformatics analysis,subcellu-lar structure isolation and localization method,construction of shRNA lentiviral vector and cell transfection,Western blot,real-time fluorescence quantitative PCR,kinetic analysis of RNA synthesis and degradation,and mitochondrial DNA immunoprecipitation-quantitative PCR analysis were utilized to explore the mechanism by which targeting HMGCS1 regulates mi-tochondrial gene expression in CC cells by exploring the mechanism of action of targeting HMGCS1.Results:HMGCS1 expres-sion is up-regulated in CC and predicts poor prognosis;HMGCS1 is mainly differentially expressed in mitochondria;knockdown of HMGCS1 leads to a general down-regulation of the mitochondrial gene expression level in CC cells;knockdown of HMGCS1 may inhibit mitochondrial gene expression by decreasing the level of mitochondrial mRNA synthesis;HMGCS1 may promote the mitochondrial gene transcription of CC cells by regulator binding to mitochondrial DNA(mtDNA)to safeguard the normal tran-scription of mitochondrial genes.Conclusion:HMGCS1 is highly expressed in CC and is associated with poor prognosis.Down-regulated HMGCS1 inhibits mitochondrial gene expression by decreasing the level of mitochondrial mRNA synthesis in CC cells,and HMGCS1 can safeguard the normal transcription of mitochondrial genes by promoting the binding of mitochondrial gene transcriptional regulators to mtDNA in CC cells.Therefore,targeted inhibition of HMGCS1 to regulate mitochondrial gene ex-pression may be a promising new strategy for CC treatment.关键词
宫颈癌/HMGCS1/线粒体基因/表达调控Key words
Cervical cancer/HMGCS1/Mitochondrial gene/Expression regulation分类
医药卫生引用本文复制引用
尚丹丹,张晓敏,杨雯萱,陈博涵,刘萍,周超..靶向HMGCS1探讨对宫颈癌细胞线粒体基因表达调控的作用机制[J].海南医科大学学报,2026,32(2):137-147,11.基金项目
This study was supported by the Shandong Province Medicine and Health Science and Technology Project(202405010551) （202405010551）
Natural Science Foundation of Hainan Province(821RC732) （821RC732）
Shandong Province Traditional Chinese Medicine Science and Technology Project(2020M085) （2020M085）
Binzhou Medical University"Clinical+X"Project(BY2021LCX26) 山东省医药卫生科技项目(202405010551) （BY2021LCX26）
海南省自然科学基金高层次人才基金项目(821RC732) （821RC732）
山东省中医药科技项目(2020M085) （2020M085）
滨州医学院"临床+X"项目(BY2021LCX26) （BY2021LCX26）
