华北农学报2025，Vol.40Issue(z1)：1-8,8.DOI:10.7668/hbnxb.20194930
基于全基因组重测序的香菇菌株遗传多样性及群体结构分析
Analysis of Population Diversity and Structure of Lentinula edodes Strains Based on Whole Genome Resequencing Data
摘要
Abstract
To investigate the genetic evolution and molecular differences of Lentinula edodes germplasm re-sources and their hybrid progeny,it utilized whole-genome resequencing technology and analyzed genetic diversity and population structure based on single-nucleotide polymorphism(SNP)data.It involved eight main cultivated vari-eties,six hybrid new varieties,and two wild strains of L.edodes.The results revealed that the SNP numbers in the wild strains Y5 and Y6 were significantly higher than those in the cultivated varieties,with 158 659 and 149 422 SNPs,respectively,and they clustered in independent branches based on genetic distance.In contrast,the cultivated varieties and their hybrid progeny exhibited similar SNP numbers and genetic relationships,with SNP counts ranging from 97 295 to 105 627 and genetic distances ranging from 0.001 2 to 0.055 3,clustering within the same branch.The branches of the cultivated varieties and their hybrid progeny could be divided into six closely related groups.These included early-maturing varieties L18 and 868,early-maturing white-faced varieties RX11,QK212,and 0912,medium-maturing firm-textured varieties 808,LX1,and 168,hybrid progeny varieties JX15 and JX3 of 808,L18,and 868,hybrid progeny varieties JXB5 and JXB15 of JX15 and JX3,and hybrid progeny varieties E14 and E36 of 0912 and JXB5.Population structure and principal component analysis revealed that L.edodes germplasm resources could be divided into four subgroups.The first class included varieties RX11,QK212,and 0912,as well as their hy-brid progeny E14 and E36.The second class consisted of main cultivated varieties LX1,168,and 808,along with their hybrid derivatives JX15 and JX3,which share extremely similar genetic information or background.The third class comprised L18 and 868,along with their multiple-generation,genetically improved,high-quality hybrid strains,JXB5 and JXB15,which exist in a transitional state between cultivated varieties and wild strains,with a propensity towards the cultivated varieties.The fourth class was represented by wild strains YX5 and YX6,which are distinctly different from the cultivated strains.This study clarified the genetic differences among L.edodes strains,laying the foundation for the selection of parents and the pairing of hybrid combinations in subsequent hybrid breeding.关键词
香菇/全基因组重测序/遗传多样性/群体结构/单核苷酸多态性Key words
Lentinula edodes/Whole genome resequencing/Genetic diversity/Population structure/Single-nu-cleotide polymorphism分类
农业科技引用本文复制引用
姚澜,王雅囡,刘振国,马宏,刘昆昂,朱彦辉,张根伟..基于全基因组重测序的香菇菌株遗传多样性及群体结构分析[J].华北农学报,2025,40(z1):1-8,8.基金项目
河北省食用菌现代种业科技创新团队(21326315D) （21326315D）
河北省科学院科技计划项目(21310,22310,23310,25A07,23A07,24A07) （21310,22310,23310,25A07,23A07,24A07）
