华北农学报2025，Vol.40Issue(z1)：9-19,11.DOI:10.7668/hbnxb.20195959
模拟涝害胁迫下大豆生理和转录组分析
Physiological and Transcriptomic Analysis of Soybean under Waterlogging Stress
摘要
Abstract
Waterlogging stress severely impacts soybean production.To investigate the effects of waterlogging stress on soybean growth and development and elucidate its response mechanisms,this study employed four soybean germplasms cultivated in the Huaibei region as experimental materials.Through simulated waterlogging treatment at the V2 stages,combined with yield analysis,physiological indices,and transcriptomic profiling,the study explores the waterlogging tolerance mechanisms in soybean.The results demonstrated that waterlogging stress significantly reduced dry matter accumulation and nitrogen uptake efficiency in the waterlogging-sensitive cultivar Xudou 18,resulting in a yield loss of 30.23％.In contrast,the waterlogging-tolerant cultivar Huaidou 13 exhibited stronger adaptability with only a 16.77％yield loss,attributed to stabilized root nitrogen absorption and maintained root dry matter accu-mulation.Transcriptomic analysis revealed that differentially expressed genes(DEGs)in Huaidou 13 under waterlog-ging stress were significantly enriched in pathways that related to the biosynthesis of secondary metabolites,photo-synthetic systems,and antioxidant activity.Conversely,DEGs in Xudou 18 were predominantly associated with hor-mone transduction,photosynthesis,and peroxisome-related pathways.A total of 148 genotype-specific DEGs were i-dentified between cultivars with contrasting waterlogging tolerance,primarily enriched in photosynthesis,secondary metabolite biosynthesis,and fatty acid metabolism.Identified transcription factors included members of the AP2/ERF-ERF,C3H,ARR-B,NAC,and WRKY families.In summary,transcription factors or genes in secondary meta-bolic pathways that may be related to waterlogging tolerance were screened.关键词
大豆/涝害胁迫/生理代谢/转录组Key words
Soybean/Waterlogging stress/Physiological metabolism/Transcriptome分类
农业科技引用本文复制引用
赵志鑫,刘宁宁,徐海风,王亚琪,傅蒙蒙,李曙光,余希文..模拟涝害胁迫下大豆生理和转录组分析[J].华北农学报,2025,40(z1):9-19,11.基金项目
江苏省种业创新"揭榜挂帅"项目(JBGS[2021]057) （JBGS[2021]057）
生物育种钟山实验室项目(ZSBBL-KY2023-03) （ZSBBL-KY2023-03）
淮安市农业科学研究院科研发展基金项目(HNY202112) （HNY202112）
国家重点研发计划项目(2024YFD1201402) （2024YFD1201402）
