华北农学报2025，Vol.40Issue(z1)：20-28,9.DOI:10.7668/hbnxb.20194398
不同品质小麦的转录组比较分析
Comparative Analysis on the Transcriptome Profile of Wheat with Different Flour Qualities
摘要
Abstract
In order to elucidate the molecular mechanism of flour-quality regulation at the transcriptional level,wheat mutant varieties A94 and A261 were obtained by 60Co-γ irradiation mutagenesis of Shimai 15,and the develo-ping seeds at days 7,14,21 and 28 after flowering were subjected to transcriptome sequencing,and water absorp-tion,dough development time,stable time,softening degree and farinogram index of the three varieties were meas-ured.The results showed that farinogram parameters of two mutant varieties were better than those of Shimai 15.Twenty-four samples were sequenced,and after the data were filtered and annotated,a total of 26 714 genes were i-dentified.With respect to Shimai 15,there were 1 042,258,301 and 366 differentially expressed genes(DEGs)at four time points in A94,respectively,and there were 2 178,248,117 and 959 DEGs in A261,respectively.KEGG enrichment showed that DEGs were mainly involved in pathways of starch and sucrose metabolism,protein process-ing in endoplasmic reticulum.GO enrichment showed that DEGs were mainly involved in glycogen biosynthesis processes,amyloplast and other biological processes.By analyzing the differential genes in these pathways and bio-logical processes,it was hypothesized that the reasons for the improved flour quality in the mutant varieties include the up-regulation of genes related to sucrose and starch synthesis and metabolism in the early stages of filling,the degradation of misfolded proteins in the middle and late stages,and the differential expression of genes regulating protein and starch accumulation.关键词
小麦/品质/诱变/转录组/淀粉/蛋白Key words
Wheat/Flour quality/Mutagenesis/Transcriptome/Starch/Protein分类
农业科技引用本文复制引用
李亚青,张楠,彭义峰,张士昌,何明琦,史占良,李孟军..不同品质小麦的转录组比较分析[J].华北农学报,2025,40(z1):20-28,9.基金项目
河北省小麦产业技术体系项目(HBCT2023010206) （HBCT2023010206）
