河北医学2026，Vol.32Issue(1)：1-8,8.DOI:10.3969/j.issn.1006-6233.2026.01.01
LncRNA SNHG14调控miR-101-3p/SGK1轴对高糖诱导的肾小球系膜细胞增殖及纤维化的影响
The Effect of LncRNA SNHG14 on High Glucose Induced Proliferation and Fibrosis of Glomerular Mesangial Cells by Regulating the miR-101-3p/SGK1 Axis
摘要
Abstract
Objective:To explore the effect of LncRNA SNHG14 on high glucose(HG)-induced prolif-eration and fibrosis of glomerular mesangial cells(GMC)by regulating the miR-101-3p/SGK1 axis.Meth-ods:The serum of patients with diabetic nephropathy(DN)(DN group)and healthy people(NC group)were collected.RT-qPCR was used to detect the expression of SNHG14,miR-101-3p,and SGK1 mRNA in serum.Human GMC(HGMC)was cultured and assigned into NG group,HG group,HG+SNHG14 knock-down control(si-NC)group,HG+SNHG14 knockdown(si-SNHG14)group,HG+si-SNHG14+miR-101-3p inhibitor(in-miR-101-3p)group,and HG+si-SNHG14+SGK1 overexpression plasmid(pcDNA-SGK1)group.RT-qPCR was used to detect the expression of SNHG14,miR-101-3p,and SGK1 mRNA in each group.CCK-8,cloning formation,immunofluorescence,and Western blot were used to detect HGMC proliferation and fibrosis in each group.The dual luciferase and RIP assays were used to detect the relation-ship between miR-101-3p and SNHG14(or SGK1).Results:The expression levels of SNHG14 and SGK1 mRNA in serum of patients in DN group were higher than those in the NC group,while the expression of miR-101-3p was lower(P<0.05).Compared with the NG group,SNHG14 expression in the HG group was up-regulated,accompanied by significantly increased cell proliferation activity(OD value,colony formation,Ki67-positive cell rate)and elevated expression of fibrosis markers(SGK1,α-SMA,FN,Col IV),while miR-101-3p expression was downregulated(P<0.05).Importantly,compared with the HG+si-NC group,knockdown of SNHG14(HG+si-SNHG14 group)effectively reversed these HG-induced effects,significantly inhibiting cell proliferation and fibrosis(P<0.05).Furthermore,on the basis of SNHG14 knockdown,either inhibition of miR-101-3p(HG+si-SNHG14+in-miR-101-3p group)or overexpression of SGK1(HG+si-SNHG14+pcDNA-SGK1 group)could significantly reverse the anti-proliferative and anti-fibrotic effects caused by SNHG14 knockdown(P<0.05).SNHG14 targeted and regulated the miR-101-3p/SGK1 axis(P<0.05).Conclusion:LncRNA SNHG14 promotes HG induced proliferation and fibrosis of GMC by inhibiting miR-101-3p and upregulating SGK1.关键词
LncRNA SNHG14/miR-101-3p/SGK1轴/高糖/肾小球系膜细胞/纤维化Key words
LncRNA SNHG14/miR-101-3p/SGK1 axis/High glucose/Glomerular mesang-ial cells/Fibrosis引用本文复制引用
牛晓静,叶寒露,张利芳,张建..LncRNA SNHG14调控miR-101-3p/SGK1轴对高糖诱导的肾小球系膜细胞增殖及纤维化的影响[J].河北医学,2026,32(1):1-8,8.基金项目
武汉市中医药科研项目,(编号:WZ24A18) （编号:WZ24A18）
