河北医学2026，Vol.32Issue(1)：20-27,8.DOI:10.3969/j.issn.1006-6233.2026.01.04
木犀草素调控AMPK/ULK1通路对慢性萎缩性胃炎大鼠胃黏膜损伤的影响
The Effect of Luteolin on Gastric Mucosal Injury in Rats with Chronic Atrophic Gastritis via Regulation of the AMPK/ULK1 Pathway
摘要
Abstract
Objective:To explore the effect of luteolin on gastric mucosal damage in rats with chronic a-trophic gastritis by modulating the AMP-activated protein kinase(AMPK)/Unc51-like kinase 1(ULK1)pathway.Methods:A chronic atrophic gastritis rat model was established and separated into a model group,a luteolin group(80mg/kg),an inhibitor group(20mg/kg AMPK inhibitor Compound C),and a luteolin+activator group(80mg/kg luteolin and 200mg/kg AMPK activator AICAR).The control group consisted of unmodeled rats.Each group consisted of 12 samples,receiving continuous gavage administration for 12 weeks.The spectrophotometer was used to measure gastric mucosal blood flow.ELISA method was used to measure serum gastrin(GAS),plasma motilin(MTL),and levels of IL-1β,TNF-α,SOD,and MDA in gastric mucosal tissue.HE staining was used to observe the pathological morphology of gastric mucosal tissue.TUNEL staining method was used to determine the apoptosis of gastric mucosal tissue cells.Transmission elec-tron microscopy was used to observe the ultrastructure of gastric mucosa.Immunohistochemistry was used to measure the Bcl-2 protein in gastric mucosal tissue.In addition,Western blot was used to measure the AMPK/ULK1 pathway related proteins in gastric mucosal tissue.Results:Compared with the control group,the epithelial cells of the gastric mucosal tissue in the model group shed,and there was more infiltration of in-flammatory cells.The shape of the gastric mucosal wall cells was irregular,and the main cell structure was se-verely injured.The gastric mucosal blood flow was reduced,and the levels of GAS,MTL,SOD,and the ex-pression of Bcl-2 protein declined.While the pathological inflammatory score,apoptosis rate,levels of IL-1β,TNF-α,MDA,and expression of p-AMPK and p-ULK1 proteins increased(P<0.05).Compared with the model group,the gastric mucosal tissue pathological damage was prominently improved in the luteolin group and inhibitor group.The gastric mucosal blood flow,the levels of GAS,MTL,SOD,and the expression of Bcl-2 protein increased.While the pathological inflammatory score,apoptosis rate,levels of IL-1β,TNF-α,MDA,and expression of p-AMPK and p-ULK1 proteins declined(P<0.05).Compared with the luteo-lin group,the improvement effect of magnolol+activator group on the above indicators in rats can be weakened by AMPK activator(P<0.05).Conclusion:Luteolin may alleviate gastric mucosal injury in rats with chronic atrophic gastritis by inhibiting AMPK/ULK1 pathway.关键词
木犀草素/腺苷酸活化蛋白激酶/Unc51样激酶1/慢性萎缩性胃炎Key words
Luteolin/AMP-activated protein kinase/Unc51-like kinase 1/Chronic atrophic gastritis引用本文复制引用
岳华强,薛娟,计春燕,苏志威,李诗旖,丁毓雪,程豪,孙俊..木犀草素调控AMPK/ULK1通路对慢性萎缩性胃炎大鼠胃黏膜损伤的影响[J].河北医学,2026,32(1):20-27,8.基金项目
2025-2026年度湖北省中医药管理局中医药科研项目,(编号:ZY2025Q017) （编号:ZY2025Q017）
