河北医学2026，Vol.32Issue(1)：28-33,6.DOI:10.3969/j.issn.1006-6233.2026.01.05
基于"气血辨证"理论探讨白花蛇舌草对肝硬化大鼠的作用及对NF-κB表达纤维化的影响
An Investigation into the Effects of Hedyotis diffusa on Rats with Liver Cirrhosis Based on the Theory of Qi and Blood Differentiation and Its Influence on NF-κB Expression and Fibrosis
摘要
Abstract
Objective:To investigate the effects of Hedyotis diffusa on hepatic fibrosis and NF-κB ex-pression in rats with liver cirrhosis based on the theory of Qi and Blood Differentiation.Methods:The SD rats were randomly divided into a control group,a model group,a positive drug Yishanfu group,and Hedyotis dif-fusa low-and high-dose groups,with 7 rats in each group.Intraperitoneal injection of carbon tetrachloride was conducted to construct a rat model of liver cirrhosis.HE staining was used to detect pathological changes in liver tissue.ELISA assay was used to detect the levels of alanine aminotransferase(ALT),aspartate amin-otransferase(AST),total bilirubin(TBIL),IL-6,and TNF-α in serum.Masson staining was used to detect the degree of fibrosis in liver tissue.RT-qPCR was used to detect the mRNA expressions of α-SMA,Col1a1,and TGF-β1 in liver tissues.An immunohistochemical staining assay was used to detect the expression and lo-calization of p65 NF-κB protein in liver tissue.Results:Compared with the control group,rats in the model group exhibited marked pathological changes indicative of liver cirrhosis in their hepatic tissue.Serum levels of ALT,AST,TBIL,IL-6,and TNF-α were significantly elevated(P<0.05).Collagen fibre proliferation in the liver tissue was markedly increased,alongside elevated expression of α-SMA,Col1a1,TGF-β1,and p65 NF-κB in the hepatic tissue(P<0.05).Compared with the model group,pathological damage in liver tissue was improved in rats from the positive drug group and the low-and high-dose Hedyotis diffusa groups.Serum levels of ALT,AST,TBIL,IL-6,and TNF-α decreased(P<0.05),collagen fibre proliferation in liver tis-sue diminished,and liver tissue expression of α-SMA,Col1a1,TGF-β1,and p65 NF-κB in liver tissue de-creased(P<0.05).Conclusion:Hedyotis diffusa reduces fibrosis in the liver tissue of rats with cirrhosis by inhibiting inflammation,and the mechanism may be related to the inhibition of NF-κB signaling.关键词
白花蛇舌草/肝硬化/NF-κB/纤维化/炎症Key words
Hedyotis diffusa/Liver cirrhosis/NF-κB/Inflammation/Fibrosis引用本文复制引用
林燕,史国琴,孙园园..基于"气血辨证"理论探讨白花蛇舌草对肝硬化大鼠的作用及对NF-κB表达纤维化的影响[J].河北医学,2026,32(1):28-33,6.基金项目
2024年度兰州市第四批科技计划项目,(编号:2024-4-19) （编号:2024-4-19）
