河北医学2026，Vol.32Issue(1)：33-39,7.DOI:10.3969/j.issn.1006-6233.2026.01.06
基于NF-κB/NLRP3信号通路探讨生脉注射液对感染性休克大鼠急性肺损伤的影响及机制
Effects and Mechanisms of Shengmai Injection on Acute Lung Injury in Rats with Septic Shock Based on the NF-κB/NLRP3 Signaling Pathway
摘要
Abstract
Objective:To explore the effects and mechanisms of Shengmai injection on acute lung injury in rats with septic shock based on the NF-κB/NLRP3 signaling pathway.Methods:Male Sprague-Dawley(SD)rats were randomly divided into the control group,the model group,the positive drug dexamethasone group and the Shengmai injection group,with 10 rats in each group.A rat model of septic shock was construc-ted by intravenous injection of lipopolysaccharide.The serum endotoxin level of in rats in each group were de-tected.The lung coefficient and wet/dry weight ratio of rats in each group were measured.Hematoxylin-eosin(HE)staining was used to detect pathological damage in lung tissue.TUNEL staining was used to detect ap-optosis in lung tissues.The protein expression of Bcl-2,Bax,cleaved caspase-3,p-p65 NF-κB/p65 NF-κB,NLRP3,cleaved Caspase-1(caspase-1 p20)and GSDMD was detected by Western blot.The levels of IL-1β,IL-6,TNF-α in bronchoalveolar lavage fluid were detected by Enzyme-linked immunosorbent(ELISA).Results:Compared with the control group,the serum endotoxin levels of rats in the model group were increased(P<0.05),lung coefficient and wet/dry weight ratio of lung tissue increased(P<0.05),and lung tissue showed significant pathological damage,the apoptosis rate of lung histiocytes increased(P<0.05),the Bcl-2 in lung tissue decreased,while Bax,cleaved caspase-3,p-p65 NF-κB/p65 NF-κB,NLRP3,caspase-1 p20,GSDMD were increased(P<0.05),the levels of IL-1β,IL-6,TNF-α in bronchoalveolar lavage fluid increased(P<0.05).Compared with the model group,the serum endotoxin levels of the positive drug group and Shengmai injection group were decreased(P<0.05),lung coefficient and wet/dry weight ratio of lung tissue decreased(P<0.05),and pathological damage in lung tissue reduced,the apoptosis rate of lung histiocytes decreased(P<0.05),the Bcl-2 in lung tissue increased,while Bax,cleaved caspase-3,p-p65 NF-κB/p65 NF-κB,NLRP3,caspase-1 p20,GSDMD were decreased(P<0.05),the levels of IL-1β,IL-6,TNF-α in bronchoalveolar lavage fluid decreased(P<0.05).Conclusion:Shengmai injection can alle-viate lung tissue damage in rats with septic shock by inhibiting lung cell apoptosis and lung inflammation,the mechanism of which may be related to the inhibition of NF-κB/NLRP3 signaling pathway.关键词
急性肺损伤/生脉注射液/感染性休克/NF-κB/NLRP3/凋亡Key words
Acute lung injury/Shengmai injection/Septic shock/NF-κB/NLRP3/Apopto-sis引用本文复制引用
解欢琼,肖坛..基于NF-κB/NLRP3信号通路探讨生脉注射液对感染性休克大鼠急性肺损伤的影响及机制[J].河北医学,2026,32(1):33-39,7.基金项目
云南省卫生健康委临床医学中心2022-2024年建设项目,(编号:202203AF150091) （编号:202203AF150091）
