河北农业科学2025，Vol.29Issue(5)：48-53,6.DOI:10.12148/hbnykx.20250117
外源褪黑素对低温胁迫下棉花种子萌发抗氧化酶活性及渗透调节物质含量的影响
Effects of Exogenous Melatonin on Seed Germination,Antioxidant Enzyme Activity and Osmotic Regulation Substance Content of Cotton under Low Temperature Stress
摘要
Abstract
Low temperature is the main abiotic stress during cotton sowing and emergence period,significantly inhibiting cotton seed germination.Melatonin(MT)can actively regulate the stress resistance of plants.Analyz-ing the regulatory effect of MT induced treatment on cotton seed germination under low temperature stress can provide theoretical basis for the application of MT in cotton production and stress cultivation.The seeds of trans-genic insect-resistant cotton variety Jimian 30 were used as the experimental materials,and the uninitiated seeds were used as controls.Using germination poten-tial and germination rate indicators,the appropriate concentration for MT soaking treatment to alleviate low temperature(15℃)stress was screened.The re-sults showed that different concentrations of MT treat-ment significantly increased seed germination poten-tial,but had no significant effect on the seed germi-nation rate,with the highest germination potential ob-served at a concentration of 10 μ mol/L.Then a comparative experimental design was adopted,with seeds treated with MT soaking at a concentration of 10 μmol/L as the experimental group(MT)and uninitiated seeds as the control.The germination indicators(germination rate and embryonic root length)of seeds at 15℃low temperature,as well as the activities of antioxidant enzymes[superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)],osmoregulatory substances(proline and soluble sugars),and malondialdehyde(MDA)content during germination were analyzed.The results showed that MT treatment could significantly promote early seed germination and embryonic root e-longation at low temperatures,increase SOD,POD,and CAT activities,as well as proline and soluble sugar content,and reduce MDA content.The treatment with MT solution with a concentration of 10 μmol/L can ef-fectively alleviate the inhibitory effect of 15℃low temperature on cotton seed germination.关键词
棉花/褪黑素/低温胁迫/种子萌发Key words
Cotton/Melatonin/Low temperature stress/Seed germination分类
农业科技引用本文复制引用
张海娜,赵存鹏,刘素恩,耿军义,李丹,王凯辉,高鑫隆,马玉柱,郭宝生..外源褪黑素对低温胁迫下棉花种子萌发抗氧化酶活性及渗透调节物质含量的影响[J].河北农业科学,2025,29(5):48-53,6.基金项目
河北省农林科学院现代农业科技创新专项(2022KJCXZX-MHS-10) （2022KJCXZX-MHS-10）
