基础医学与临床2026，Vol.46Issue(2)：208-214,7.DOI:10.16352/j.issn.1001-6325.2026.02.0208
筋脉通对高糖诱导RSC96细胞糖酵解重编程及RAW264.7细胞极化的调节作用
Regulatory effects of Jinmaitong on high glucose-induced glycolytic reprogramming in RSC96 cells and polarization of RAW264.7 cells
摘要
Abstract
Objective To investigate the regulatory effects of Jinmaitong on glycolysis and histone lactylation in high glucose-induced rat Schwann cell line RSC96,and its impact on macrophage polarization.Methods A high-glucose metabolic stress model was established using RSC96 cells,followed by treatment with different concentra-tions of Jinmaitong.Cell proliferation,lactate levels,and the expression of glycolytic enzymes(PKM2,LDHA)and histone lactylation marker H3K18la were assessed to evaluate metabolic and epigenetic changes.In addition,conditioned media from high-glucose-treated RSC96 cells were applied to RAW264.7 macrophages to examine changes in polarization-related phenotypic markers.Results High-glucose stimulation enhanced glycolytic activity and lactate accumulation in RSC96 cells,accompanied by upregulation of H3K18la modification,indicating a state of metabolic stress.Jinmaitong treatment led to a dose-dependent reduction in glycolytic enzyme expression and lactate levels,and significantly suppressed H3K18la expression.Conditioned media from high-glucose-treated Schwann cells promoted M1 polarization of RAW264.7 cells,whereas Jinmaitong intervention reversed this trend,promoting M2 polarization and alleviating the pro-inflammatory microenvironment.Conclusions High-glu-cose conditions induce glycolytic reprogramming and histone lactylation in Schwann cells,which may contribute to macrophage polarization and neural injury.关键词
糖酵解重编程/组蛋白乳酸化/巨噬细胞极化/筋脉通/糖尿病周围神经病变Key words
glycolytic reprogramming/histone lactylation/macrophage polarization/Jinmaitong(JMT)/diabetic peripheral neu-ropathy分类
医药卫生引用本文复制引用
车彦霏,覃东汕,余姿漫,王皓宇,杨丹,梁晓春..筋脉通对高糖诱导RSC96细胞糖酵解重编程及RAW264.7细胞极化的调节作用[J].基础医学与临床,2026,46(2):208-214,7.基金项目
国家自然科学基金(82004182) （82004182）
